中文版 | English
Title

Ferrous sulfate-loaded hydrogel cures Staphylococcus aureus infection via facilitating a ferroptosis-like bacterial cell death in a mouse keratitis model

Author
Corresponding AuthorHu,Liangbin; Zhou,Xiaohui
Publication Years
2022-11-01
DOI
Source Title
ISSN
0142-9612
EISSN
1878-5905
Volume290
Abstract
Hydrogels loaded with ampicillin, vancomycin or other antibiotics are one of the most widely used therapeutic agents for keratitis caused by Staphylococcus aureus. However, emergence of methicillin-resistant S. aureus (MRSA) makes infections harder to be treated by antibiotic-based hydrogels, urging the development of novel antibacterial materials. Inspired by mammalian ferroptosis, we determined the bactericidal effects of ferrous sulfate (FeSO) on S. aureus, and evaluated the therapeutic potential of FeSO-loaded hydrogel in a mouse keratitis model. The results showed that FeSO facilitated ferroptosis-like cell death in S. aureus with the key characteristics of reactive oxygen species (ROS) generation and lipid peroxidation. Notably, FeSO also efficiently killed persisters and MRSA, and eliminated biofilms of S. aureus. RNA profiles demonstrated that ferroptosis-related genes were significantly up-regulated, and the genes responsible for cell wall and cell membrane biosynthesis were down-regulated after exposure to Fe, supporting the occurrence of ferroptosis and cell lysis. We further prepared a FeSO-loaded hydrogel by using hyaluronic acid (HA) and ascorbate. The FeSO hydrogel has the characteristics of injectability, self-healing, uniform distribution of Fe in the three-dimensional gel structure, appropriate fluidity, high-water retention, high efficacy to kill MRSA, and excellent biocompatibility. In a mouse keratitis model, we showed that treatment of animals with FeSO hydrogel led to a rapid recovery of from keratitis, prevented the dissimilation of MRSA to the lung, and alleviated systemic inflammation, demonstrating the therapeutic potential of FeSO hydrogel. Taken together, our results indicated that FeSO hydrogel is a promising alternative to current antibiotics-dependent therapeutic materials for the treatment of infections by MRSA.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Key Research and Development Program of China[2019YFC1606302];National Natural Science Foundation of China[31901795];National Natural Science Foundation of China[32172321];
WOS Research Area
Engineering ; Materials Science
WOS Subject
Engineering, Biomedical ; Materials Science, Biomaterials
WOS Accession No
WOS:000867565000003
Publisher
ESI Research Field
MATERIALS SCIENCE
Scopus EID
2-s2.0-85139291784
Data Source
Scopus
Citation statistics
Cited Times [WOS]:6
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/406173
DepartmentSchool of Public Health and Emergency Management
Affiliation
1.School of Food and Biological Engineering,Shaanxi University of Science and Technology,Xi'an,China
2.School of Food Science,Henan Institute of Science and Technology,Xinxiang,China
3.School of Chemical Engineering,Yeungnam University,Gyeongsan,280 Daehak-Ro, Gyeongbuk,South Korea
4.College of Animal Science and Veterinary Medicine,Henan Institute of Science and Technology,Xinxiang,China
5.School of Public Health and Emergency Management,Southern University of Science and Technology,ShenZhen,Guangdong,China
Corresponding Author AffilicationSchool of Public Health and Emergency Management
Recommended Citation
GB/T 7714
Wang,Zhen,Li,Hongbo,Zhou,Wei,et al. Ferrous sulfate-loaded hydrogel cures Staphylococcus aureus infection via facilitating a ferroptosis-like bacterial cell death in a mouse keratitis model[J]. BIOMATERIALS,2022,290.
APA
Wang,Zhen.,Li,Hongbo.,Zhou,Wei.,Lee,Jintae.,Liu,Zhenbin.,...&Zhou,Xiaohui.(2022).Ferrous sulfate-loaded hydrogel cures Staphylococcus aureus infection via facilitating a ferroptosis-like bacterial cell death in a mouse keratitis model.BIOMATERIALS,290.
MLA
Wang,Zhen,et al."Ferrous sulfate-loaded hydrogel cures Staphylococcus aureus infection via facilitating a ferroptosis-like bacterial cell death in a mouse keratitis model".BIOMATERIALS 290(2022).
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