中文版 | English
Title

Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages

Author
Corresponding AuthorWang, Zhaoqin; Zhang, Guoliang; Lu, Shuihua
Publication Years
2022-10-17
DOI
Source Title
ISSN
1471-2180
Volume22Issue:1
Abstract
Background Tuberculosis (TB) caused by Mycobacterium tuberculosis (M. tb) remains a global health issue. The characterized virulent M. tb H37Rv, avirulent M. tb H37Ra and BCG strains are widely used as reference strains to investigate the mechanism of TB pathogenicity. Here, we attempted to determine metabolomic signatures associated with the Mycobacterial virulence in human macrophages through comparison of metabolite profile in THP-1-derived macrophages following exposure to the M. tb H37Rv, M. tb H37Ra and BCG strains. Results Our findings revealed remarkably changed metabolites in infected macrophages compared to uninfected macrophages. H37Rv infection specifically induced 247 differentially changed metabolites compared to H37Ra or BCG infection. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed H37Rv specifically induces tryptophan metabolism. Moreover, quantitative PCR (qPCR) results showed that indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase 2 (TDO2) which converts the tryptophan to a series of biologically second metabolites were up-regulated in H37Rv-infected macrophages compared to H37Ra- or BCG-infected macrophages, confirming the result of enhanced tryptophan metabolism induced by H37Rv infection. These findings indicated that targeting tryptophan (Trp) metabolism may be a potential therapeutic strategy for pulmonary TB. Conclusions We identified a number of differentially changed metabolites that specifically induced in H37Rv infected macrophages. These signatures may be associated with the Mycobacterial virulence in human macrophages. The present findings provide a better understanding of the host response associated with the virulence of the Mtb strain.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
National Key Research and Development Plan["2021YFA1300902","2020YFA0907201"] ; National Natural Science Foundation of China[82170009] ; Guangdong Scientific and Technological Foundation["2019B1515120041","2020B1111170014","2020A1515010977"] ; Shenzhen Scientific and Technological Foundation[KCXFZ202002011007083]
WOS Research Area
Microbiology
WOS Subject
Microbiology
WOS Accession No
WOS:000869251300001
Publisher
ESI Research Field
MICROBIOLOGY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/406504
DepartmentThe Third People's Hospital of Shenzhen
南方科技大学第一附属医院
Affiliation
1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Guangdong Prov Clin Res Ctr TB, Natl Clin Res Ctr Infect Dis, Shenzhen 518112, Peoples R China
2.Guangdong Med Univ, Sch Basic Med Sci, Dongguan, Peoples R China
First Author AffilicationThe Third People's Hospital of Shenzhen;  Shenzhen People's Hospital
Corresponding Author AffilicationThe Third People's Hospital of Shenzhen;  Shenzhen People's Hospital
First Author's First AffilicationThe Third People's Hospital of Shenzhen;  Shenzhen People's Hospital
Recommended Citation
GB/T 7714
Xiao, Guohui,Zhang, Su,Zhang, Like,et al. Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages[J]. BMC MICROBIOLOGY,2022,22(1).
APA
Xiao, Guohui.,Zhang, Su.,Zhang, Like.,Liu, Shuyan.,Li, Guobao.,...&Lu, Shuihua.(2022).Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages.BMC MICROBIOLOGY,22(1).
MLA
Xiao, Guohui,et al."Untargeted metabolomics analysis reveals Mycobacterium tuberculosis strain H37Rv specifically induces tryptophan metabolism in human macrophages".BMC MICROBIOLOGY 22.1(2022).
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