Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use

Luengwilai, Kietsuda1,3; Yu, Jingwei1,4; Jimenez, Randi C.1,5; Thitisaksakul, Maysaya1,6; Vega, Andrea2; Dong, Shaoyun1,7; Beckles, Diane M.1

2022-10-01

10.3390/ijms231911229

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Impact Factor & Quartile

1422-0067

A large collection of transgenic tomato lines, each ectopically expressing a different Arabidopsis thaliana transcription factor, was screened for variants with alterations in leaf starch. Such lines may be affected in carbon partitioning, and in allocation to the sinks. We focused on 'L4080', which harbored an A. thaliana zDof (DNA-binding one zinc finger) isoform 1.3 (AtzDof1.3) gene, and which had a 2-4-fold higher starch-to-sucrose ratio in source leaves over the diel (p < 0.05). Our aim was to determine whether there were associated effects on productivity. L4080 plants were altered in nitrogen (N) and carbon (C) metabolism. The N-to-C ratio was higher in six-week-old L4080, and when treated with 1/10 N, L4080 growth was less inhibited compared to the wild-type and this was accompanied by faster root elongation (p < 0.05). The six-week-old L4080 acquired 42% more dry matter at 720 ppm CO2, compared to ambient CO2 (p < 0.05), while the wild-type (WT) remained unchanged. GC-MS-TOF data showed that L4080 source leaves were enriched in amino acids compared to the WT, and at 49 DPA, fruit had 25% greater mass, higher sucrose, and increased yield (25%; p < 0.05) compared to the WT. An Affymetrix cDNA array analysis suggested that only 0.39% of the 9000 cDNAs were altered by 1.5-fold (p < 0.01) in L4080 source leaves. C-14-labeling of fruit disks identified potential differences in 14-DPA fruit metabolism suggesting that post-transcriptional regulation was important. We conclude that AtzDof1.3 and the germplasm derived therefrom, should be investigated for their 'climate-change adaptive' potential.

Dof transcription factor tomato carbohydrates nitrogen metabolism

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English

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UC BioSTAR, National Science Foundation[MCB 6011233] ; USDA CSREES CRIS[CA-D*-PLS-7821-H] ; ANID-FONDECYT[1211894] ; ANID-Millennium Science Initiative Program[NCN2021_010] ; ANID PIA/BASAL[FB0002] ; CONICYT FONDECYT[117163]

Biochemistry & Molecular Biology ; Chemistry

Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary

WOS:000867764100001

MDPI

CHEMISTRY

Web of Science

1.Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA
2.Univ Adolfo Ibanez, Fac Engn & Sci, Ctr Appl Ecol & Sustainabil CAPES, Millennium Nucleus Dev Super Adaptable Plants MN, Santiago 7941169, Chile
3.Kasetsart Univ, Fac Agr Kamphaeng Saen, Dept Hort Dept, Kamphaeng Saen Campus, Kamphaeng Saen 73140, Nakhon Pathom, Thailand
4.Southern Univ Sci & Technol, Sch Life Sci, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Peoples R China
5.Calif Dept Food Agr, 3294 Meadowview Rd, Sacramento, CA 95832 USA
6.Khon Kaen Univ, Fac Sci, Dept Biochem, Khon Kaen 40002, Thailand
7.Chinese Acad Agr Sci, Inst Vegetable & Flowers, Beijing 10008, Peoples R China

Luengwilai, Kietsuda,Yu, Jingwei,Jimenez, Randi C.,et al. Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(19).

Luengwilai, Kietsuda.,Yu, Jingwei.,Jimenez, Randi C..,Thitisaksakul, Maysaya.,Vega, Andrea.,...&Beckles, Diane M..(2022).Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(19).

Luengwilai, Kietsuda,et al."Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.19(2022).