Luteolin Inhibited the Self-Renewal and Altered the Polarization of Primary Alveolar Macrophages

Geng, Aiai1,2; Chen, Shanze3; Ren, Laibin3; Zhao, Xueyi2; Pei, Tianli1; Jia, Bo1; Gong, Daoyin4

2022-08-18

10.1155/2022/3517020

Contrast Media & Molecular Imaging   Impact Factor & Quartile

1555-4309

1555-4317

Pure plant extract luteolin has been demonstrated to possess numerous biological and immunological effects. However, how luteolin affects mice alveolar macrophages' self-renewal and polarization closely related to inflammatory and immunomodulatory is still unknown. In our study, the transcriptomic analysis showed that several self-renewal-related pathways in luteolin-pretreated alveolar macrophages were inhibited compared to the granulocyte-macrophage colony-stimulating factor (GM-CSF)-treated group. Ki-67 staining and EdU assay indicated that luteolin inhibited GM-CSF-induced alveolar macrophage proliferation. Moreover, GM-CSF-induced expressions of c-Myc and KLF4 were significantly suppressed by luteolin at transcriptional and protein levels. Besides, we found that luteolin promoted M1 macrophage polarization induced by LPS plus IFN-gamma. At the same time, it inhibited M2 macrophage polarization induced by IL-4 in both alveolar and bone marrow-derived macrophages by detecting macrophage polarization-related gene expressions at mRNA and protein levels. We found that luteolin inhibited self-renewal and altered the polarization of primary alveolar macrophages. Taken together, our data will aid in a better understanding of the immunomodulatory effects of luteolin on the primary alveolar macrophages.

SCI

English

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General project of National Natural Science Foundation of China[8187142249]

Radiology, Nuclear Medicine & Medical Imaging

Radiology, Nuclear Medicine & Medical Imaging

WOS:000867389600002

WILEY-HINDAWI

Web of Science

1.Chengdu Univ Tradit Chinese Med, Sch Basic Med Sci, Chengdu 611137, Peoples R China
2.Shanxi Univ Tradit Chinese Med, Affiliated Hosp, Taiyuan 030024, Peoples R China
3.Southern Univ Sci & Technol, Inst Resp Dis, Affiliated Hosp 1, Shenzhen Peoples Hosp, Shenzhen 518020, Peoples R China
4.Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610075, Peoples R China

Geng, Aiai,Chen, Shanze,Ren, Laibin,et al. Luteolin Inhibited the Self-Renewal and Altered the Polarization of Primary Alveolar Macrophages[J]. Contrast Media & Molecular Imaging,2022,2022.

Geng, Aiai.,Chen, Shanze.,Ren, Laibin.,Zhao, Xueyi.,Pei, Tianli.,...&Gong, Daoyin.(2022).Luteolin Inhibited the Self-Renewal and Altered the Polarization of Primary Alveolar Macrophages.Contrast Media & Molecular Imaging,2022.

Geng, Aiai,et al."Luteolin Inhibited the Self-Renewal and Altered the Polarization of Primary Alveolar Macrophages".Contrast Media & Molecular Imaging 2022(2022).