| Title | Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes |
| Author | |
| Corresponding Author | Xi, Peng |
| Publication Years | 2022-10-10
|
| DOI | |
| Source Title | |
| ISSN | 2095-5545
|
| EISSN | 2047-7538
|
| Volume | 11Issue:1 |
| Abstract | A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single photon counting, and drift stabilization, the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference, and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX's, the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications. |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | Corresponding
|
| WOS Research Area | Optics
|
| WOS Subject | Optics
|
| WOS Accession No | WOS:000865747500001
|
| Publisher | |
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:0
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/406539 |
| Department | Department of Biomedical Engineering 工学院 |
| Affiliation | 1.Peking Univ, Coll Future Technol, Dept Biomed Engn, Beijing 100871, Peoples R China 2.Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA 3.Emory Univ, Atlanta, GA 30332 USA 4.Southern Univ Sci & Technol, Coll Engn, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 5.Peking Univ, Natl Biomed Imaging Ctr, Beijing 100871, Peoples R China |
| First Author Affilication | Department of Biomedical Engineering; College of Engineering |
| Corresponding Author Affilication | Department of Biomedical Engineering; College of Engineering |
| Recommended Citation GB/T 7714 |
Xu, Xinzhu,Jia, Shu,Xi, Peng. Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes[J]. Light:Science & Applications,2022,11(1).
|
| APA |
Xu, Xinzhu,Jia, Shu,&Xi, Peng.(2022).Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes.Light:Science & Applications,11(1).
|
| MLA |
Xu, Xinzhu,et al."Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes".Light:Science & Applications 11.1(2022).
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