Two Rac1 pools integrate the direction and coordination of collective cell migration

Zhou，Sijia1; Li，Peng2; Liu，Jiaying1,3; Liao，Juan4; Li，Hao1; Chen，Lin3; Li，Zhihua5; Guo，Qiongyu5; Belguise，Karine1; Yi，Bin3; Wang，Xiaobo1

2022-12-01

10.1038/s41467-022-33727-6

Nature Communications   Impact Factor & Quartile

2041-1723

Integration of collective cell direction and coordination is believed to ensure collective guidance for efficient movement. Previous studies demonstrated that chemokine receptors PVR and EGFR govern a gradient of Rac1 activity essential for collective guidance of Drosophila border cells, whose mechanistic insight is unknown. By monitoring and manipulating subcellular Rac1 activity, here we reveal two switchable Rac1 pools at border cell protrusions and supracellular cables, two important structures responsible for direction and coordination. Rac1 and Rho1 form a positive feedback loop that guides mechanical coupling at cables to achieve migration coordination. Rac1 cooperates with Cdc42 to control protrusion growth for migration direction, as well as to regulate the protrusion-cable exchange, linking direction and coordination. PVR and EGFR guide correct Rac1 activity distribution at protrusions and cables. Therefore, our studies emphasize the existence of a balance between two Rac1 pools, rather than a Rac1 activity gradient, as an integrator for the direction and coordination of collective cell migration.

English

NI Journal Papers ; NI论文

Others

National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[81971764];National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[82070630];National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[82170634];Fondation ARC pour la Recherche sur le Cancer[PJA20171206526];Fondation ARC pour la Recherche sur le Cancer[PJA20191209714];

2-s2.0-85139766335

Scopus

1.Molecular,Cellular and Developmental Biology Department (MCD),Centre de Biologie Integrative (CBI),University of Toulouse,CNRS,UPS,Toulouse,France
2.Department of Anaesthesiology,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu,China
3.Department of Anaesthesiology,Southwest Hospital,The Third Military Medical University (Army Medical University),Chongqing,China
4.Department of Stomatology,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu,China
5.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China

Zhou，Sijia,Li，Peng,Liu，Jiaying,et al. Two Rac1 pools integrate the direction and coordination of collective cell migration[J]. Nature Communications,2022,13(1).

Zhou，Sijia.,Li，Peng.,Liu，Jiaying.,Liao，Juan.,Li，Hao.,...&Wang，Xiaobo.(2022).Two Rac1 pools integrate the direction and coordination of collective cell migration.Nature Communications,13(1).

Zhou，Sijia,et al."Two Rac1 pools integrate the direction and coordination of collective cell migration".Nature Communications 13.1(2022).