Long-term potentiation reconstituted with an artificial TARP/PSD-95 complex

Ravi，Anagh Sinha1; Zeng，Menglong2; Chen，Xudong2; Sandoval，Gerardo3,4; Diaz-Alonso，Javier3,4; Zhang，Mingjie2,5,6; Nicoll，Roger A.1

2022-10-11

10.1016/j.celrep.2022.111483

Cell Reports   Impact Factor & Quartile

2211-1247

2211-1247

The critical role of AMPA receptor (AMPAR) trafficking in long-term potentiation (LTP) of excitatory synaptic transmission is now well established, but the underlying molecular mechanism is still uncertain. Recent research suggests that PSD-95 captures AMPARs via an interaction with the AMPAR auxiliary subunits—transmembrane AMPAR regulatory proteins (TARPs). To determine if such interaction is a core minimal component of the AMPAR trafficking and LTP mechanism, we engineered artificial binding partners, which individually were biochemically and functionally dead but which, when expressed together, rescue binding and both basal synaptic transmission and LTP. These findings establish the TARP/PSD-95 complex as an essential interaction underlying AMPAR trafficking and LTP.

AMPA receptor trafficking AMPAR CP: Neuroscience GluA1 LTP MAGUK postsynaptic density PSD-95 synaptic transmission TARP

SCI

English

Others

Shenzhen Graduate School, Peking University[S201101002];

Cell Biology

Cell Biology

WOS:000879830500009

CELL PRESS

2-s2.0-85139736438

Scopus

1.Department of Cellular and Molecular Pharmacology,University of California at San Francisco,San Francisco,United States
2.Division of Life Science,Hong Kong University of Science and Technology,Hong Kong,Clear Water Bay, Kowloon,Hong Kong
3.Department of Anatomy and Neurobiology,University of California at Irvine,Irvine,United States
4.Center for the Neurobiology of Learning and Memory,University of California at Irvine,Irvine,United States
5.Greater Bay Biomedical Innocenter,Shenzhen Bay Laboratory,Shenzhen,518036,China
6.School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China

Ravi，Anagh Sinha,Zeng，Menglong,Chen，Xudong,et al. Long-term potentiation reconstituted with an artificial TARP/PSD-95 complex[J]. Cell Reports,2022,41(2).

Ravi，Anagh Sinha.,Zeng，Menglong.,Chen，Xudong.,Sandoval，Gerardo.,Diaz-Alonso，Javier.,...&Nicoll，Roger A..(2022).Long-term potentiation reconstituted with an artificial TARP/PSD-95 complex.Cell Reports,41(2).

Ravi，Anagh Sinha,et al."Long-term potentiation reconstituted with an artificial TARP/PSD-95 complex".Cell Reports 41.2(2022).