Classification and Prognostic Characteristics of Hepatocellular Carcinoma Based on Glycolysis Cholesterol Synthesis Axis

Deng, Weijie1; Zhu, Peng2; Xu, Huixuan3; Hou, Xianliang3; Chen, Wenbiao4,5

2022-09-30

10.1155/2022/2014625

Journal of Oncology   Impact Factor & Quartile

1687-8450

1687-8469

Background. Liver hepatocellular carcinoma (LIHC) is among the most frequent causes of cancer-related death across the world with a considerably poor prognosis. The current study targeted providing a new type of LIHC from the perspective of the glycolysis/cholesterol synthesis axis, predicting its prognostic characteristics, and exploring the potential role and mechanism of the glycolysis/cholesterol synthesis axis in the occurrence and development of LIHC. Methods. Based on the two expression profile data and clinical information of LIHC in The Cancer Genome Atlas (TCGA) database and hepatocellular carcinoma database (HCCDB), as well as glycolysis/cholesterol-related genes from the Molecular Signatures Database (MSigDB), unsupervised consistent clustering method was used to identify molecular subtypes. In addition, the differential genes were identified by limma package, and then the gene set was enriched, analyzed, and annotated by WebGestaltR package. At the same time, the immune infiltration analysis of tumor samples was carried out using the ESTIMATE to evaluate the tumor immune score of the samples. Finally, the differences in clinical characteristics among molecular subtypes were measured using univariate and multivariate Cox analyses. Results. According to the median standardized expression levels of glycolysis/cholesterol production genes, samples were divided into four groups (molecular subtypes): Quiescent group, Glycolysis group, Cholesterol group, and Mixed group. Significant prognostic differences were observed among the four groups. In both TCGA and HCCDB18 datasets, the prognosis of subtype Mixed was the worst, while Quiescent had a good prognosis. Cell cycle and oncogenic pathways were significantly enriched in the Mixed group. In addition, glycolysis and cholesterol production gene expressions were related to the prognostic LIHC subtype classification genes' expression levels. Conclusion. Metabolic classification regarding glycolysis and cholesterol production pathways provided new insights into the biological aspects of LIHC molecular subtypes and might help to develop personalized therapies for unique tumor metabolic profiles.

SCI

English

Others

Shenzhen Science and Technology[JCYJ20180306140810282] ; China Postdoctoral Foundation[2021M703372] ; Basic and Applied Basic Research Fund of Guangdong Province[2021A1515110967] ; Medical Research Fund of Guangdong[B2022103]

Oncology

Oncology

WOS:000868325800005

HINDAWI LTD

Web of Science

1.Southern Med Univ, Shenzhen Pingshan Dist Peoples Hosp, Pingshan Gen Hosp, Clin Skills Ctr, Shenzhen 518000, Peoples R China
2.Southern Med Univ, Shenzhen Pingshan Dist Peoples Hosp, Pingshan Gen Hosp, Cent Lab, Shenzhen 518000, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Clin Med,Res Ctr,Clin Med Coll 2,Jinan Univ, Shenzhen 518000, Peoples R China
4.Southern Med Univ, Peoples Hosp Longhua, Cent Mol Lab, Affiliated Hosp, Shenzhen 518000, Peoples R China
5.Southern Med Univ, Peoples Hosp Longhua, Dept Resp Med, Affiliated Hosp, Shenzhen 518000, Peoples R China

Deng, Weijie,Zhu, Peng,Xu, Huixuan,et al. Classification and Prognostic Characteristics of Hepatocellular Carcinoma Based on Glycolysis Cholesterol Synthesis Axis[J]. Journal of Oncology,2022,2022.

Deng, Weijie,Zhu, Peng,Xu, Huixuan,Hou, Xianliang,&Chen, Wenbiao.(2022).Classification and Prognostic Characteristics of Hepatocellular Carcinoma Based on Glycolysis Cholesterol Synthesis Axis.Journal of Oncology,2022.

Deng, Weijie,et al."Classification and Prognostic Characteristics of Hepatocellular Carcinoma Based on Glycolysis Cholesterol Synthesis Axis".Journal of Oncology 2022(2022).