A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model

Yu, Yangyi1,2; Fischenich, Kristine M.1,3; Schoonraad, Sarah A.4; Weatherford, Shane1; Uzcategui, Asais Camila4; Eckstein, Kevin3; Muralidharan, Archish4; Crespo-Cuevas, Victor3; Rodriguez-Fontan, Francisco1; Killgore, Jason P.5; Li, Guangheng2; McLeod, Robert R.4,6; Miller, Nancy Hadley1,7; Ferguson, Virginia L.3,4,8; Bryant, Stephanie J.4,8,9; Payne, Karin A.1,10

2022-10-19

10.1038/s41536-022-00256-1

NPJ REGENERATIVE MEDICINE   Impact Factor & Quartile

2057-3995

Growth plate injuries affecting the pediatric population may cause unwanted bony repair tissue that leads to abnormal bone elongation. Clinical treatment involves bony bar resection and implantation of an interpositional material, but success is limited and the bony bar often reforms. No treatment attempts to regenerate the growth plate cartilage. Herein we develop a 3D printed growth plate mimetic composite as a potential regenerative medicine approach with the goal of preventing limb length discrepancies and inducing cartilage regeneration. A poly(ethylene glycol)-based resin was used with digital light processing to 3D print a mechanical support structure infilled with a soft cartilage-mimetic hydrogel containing chondrogenic cues. Our biomimetic composite has similar mechanical properties to native rabbit growth plate and induced chondrogenic differentiation of rabbit mesenchymal stromal cells in vitro. We evaluated its efficacy as a regenerative interpositional material applied after bony bar resection in a rabbit model of growth plate injury. Radiographic imaging was used to monitor limb length and tibial plateau angle, microcomputed tomography assessed bone morphology, and histology characterized the repair tissue that formed. Our 3D printed growth plate mimetic composite resulted in improved tibial lengthening compared to an untreated control, cartilage-mimetic hydrogel only condition, and a fat graft. However, in vivo the 3D printed growth plate mimetic composite did not show cartilage regeneration within the construct histologically. Nevertheless, this study demonstrates the feasibility of a 3D printed biomimetic composite to improve limb lengthening, a key functional outcome, supporting its further investigation as a treatment for growth plate injuries.

SCI

English

Others

Eunice Kennedy Shriver National Institute of Child Health & Human Development of the National Institutes of Health (NIH)[R33HD090696] ; graduate NIH Training Grant in Molecular Biophysics[T32 GM065103]

Cell Biology ; Engineering

Cell & Tissue Engineering ; Engineering, Biomedical

WOS:000870270000001

NATURE PORTFOLIO

Web of Science

1.Univ Colorado, Colorado Program Musculoskeletal Res, Dept Orthoped, Anschutz Med Campus, Aurora, CO 80045 USA
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ,Shenzhen Key Lab Musculoskeletal Tissu, Clin Med Coll 2,Shenzhen Peoples Hosp,Dept Bone &, Shenzhen, Peoples R China
3.Univ Colorado, Dept Mech Engn, Boulder, CO 80309 USA
4.Univ Colorado, Mat Sci & Engn Program, Boulder, CO 80309 USA
5.NIST, Appl Chem & Mat Div 647, Boulder, CO USA
6.Univ Colorado, Dept Elect Comp & Energy Engn, Boulder, CO 80309 USA
7.Childrens Hosp Colorado, Musculoskeletal Res Ctr, Aurora, CO USA
8.Univ Colorado, BioFrontiers Inst, Boulder, CO 80309 USA
9.Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA
10.Univ Colorado, Gates Ctr Regenerat Med, Anschutz Med Campus, Aurora, CO 80045 USA

Yu, Yangyi,Fischenich, Kristine M.,Schoonraad, Sarah A.,et al. A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model[J]. NPJ REGENERATIVE MEDICINE,2022,7(1).

Yu, Yangyi.,Fischenich, Kristine M..,Schoonraad, Sarah A..,Weatherford, Shane.,Uzcategui, Asais Camila.,...&Payne, Karin A..(2022).A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model.NPJ REGENERATIVE MEDICINE,7(1).

Yu, Yangyi,et al."A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model".NPJ REGENERATIVE MEDICINE 7.1(2022).