中文版 | English
Title

Biofilm stratification in counter-diffused membrane biofilm bioreactors (MBfRs) for aerobic methane oxidation coupled to aerobic/anoxic denitrification: Effect of oxygen pressure

Author
Publication Years
2022-11-01
DOI
Source Title
ISSN
0043-1354
EISSN
1879-2448
Volume226
Abstract

Aerobic methane oxidation coupled with denitrification (AME-D) executed in membrane biofilm bioreactors (MBfRs) provides a high promise for simultaneously mitigating methane (CH) emissions and removing nitrate in wastewater. However, systematically experimental investigation on how oxygen partial pressure affects the development and characteristics of counter-diffusional biofilm, as well as its spatial stratification profiles, and the cooperative interaction of the biofilm microbes, is still absent. In this study, we combined Optical Coherence Tomography (OCT) with Confocal Laser Scanning Microscopy (CLSM) to in-situ characterize the development of counter-diffusion biofilm in the MBfR for the first time. It was revealed that oxygen partial pressure onto the MBfR was capable of manipulating biofilm thickness and spatial stratification, and then managing the distribution of functional microbes. With the optimized oxygen partial pressure of 5.5 psig (25% oxygen content), the manipulated counter-diffusional biofilm in the AME-D process obtained the highest denitrification efficiency, due mainly to that this biofilm had the proper dynamic balance between the aerobic-layer and anoxic-layer where suitable O gradient and sufficient aerobic methanotrophs were achieved in aerobic-layer to favor methane oxidation, and complete O depletion and accessible organic sources were kept to avoid constraining denitrification activity in anoxic-layer. By using metagenome analysis and Fluorescence in situ hybridization (FISH) staining, the spatial distribution of the functional microbes within counter-diffused biofilm was successfully evidenced, and Rhodocyclaceae, one typical aerobic denitrifier, was found to survive and gradually enriched in the aerobic layer and played a key role in denitrification aerobically. This in-situ biofilm visualization and characterization evidenced directly for the first time the cooperative path of denitrification for AME-D in the counter-diffused biofilm, which involved aerobic methanotrophs, heterotrophic aerobic denitrifiers, and heterotrophic anoxic denitrifiers.

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URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
Others
Funding Project
National Natural Science Foundation of China[42277376] ; National Natural Science Foundation of China[52070053]
ESI Research Field
ENVIRONMENT/ECOLOGY
Scopus EID
2-s2.0-85140271870
Data Source
Scopus
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/406888
DepartmentSchool of Environmental Science and Engineering
Affiliation
1.School of Civil and Environmental Engineering,Harbin Institute of Technology (Shenzhen),Shenzhen,518055,China
2.Shenzhen Research Institute,The Chinese University of Hong Kong,Shenzhen,518000,China
3.School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.Instrumental Analysis Center of Shenzhen University,Shenzhen University (Xili Campus),Shenzhen,518060,China
5.State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin,150090,China
6.Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control,Shenzhen,518055,China
Recommended Citation
GB/T 7714
Lu,Jian Jiang,Zhang,Hao,Li,Weiyi,et al. Biofilm stratification in counter-diffused membrane biofilm bioreactors (MBfRs) for aerobic methane oxidation coupled to aerobic/anoxic denitrification: Effect of oxygen pressure[J]. WATER RESEARCH,2022,226.
APA
Lu,Jian Jiang.,Zhang,Hao.,Li,Weiyi.,Yi,Jun Bo.,Sun,Fei Yun.,...&Dong,Wen Yi.(2022).Biofilm stratification in counter-diffused membrane biofilm bioreactors (MBfRs) for aerobic methane oxidation coupled to aerobic/anoxic denitrification: Effect of oxygen pressure.WATER RESEARCH,226.
MLA
Lu,Jian Jiang,et al."Biofilm stratification in counter-diffused membrane biofilm bioreactors (MBfRs) for aerobic methane oxidation coupled to aerobic/anoxic denitrification: Effect of oxygen pressure".WATER RESEARCH 226(2022).
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