Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids

Julien，Maxime1,2; Zhao，Yu3; Ma，Ran3; Zhou，Youping3,4; Nakagawa，Mayuko2,5; Yamada，Keita6; Yoshida，Naohiro6,7; Remaud，Gérald S.8; Gilbert，Alexis2,5

2022-12-01

10.1016/j.orggeochem.2022.104516

ORGANIC GEOCHEMISTRY   Impact Factor & Quartile

0146-6380

1873-5290

Carbon-13 position-specific isotope analysis of fatty acids from vegetable oils was performed using Nuclear Magnetic Resonance in the present study. The measured C patterns are not totally in accordance with the conventional view of the relative C-depletion of acetogenic lipids and their alternation of C-enriched and C-depleted carbon positions. The results presented here provide a new evaluation of the isotopic fractionation associated with fatty acids biosynthesis. Whereas it is commonly acknowledged that the pyruvate dehydrogenase (PDH) is responsible for the C distribution within fatty acids, data from the present work demonstrate that the conversion of acetyl-CoA to malonyl-CoA catalyzed by acetyl-CoA carboxylase (ACC) needs to be considered while explaining the measured non-stochastic C pattern within fatty acids. These data combined with steady-state calculations give a new description of metabolic steps responsible for the typical C intramolecular distribution of acetogenic lipids. In addition, the non-stochastic pattern measured in these plant fatty acids is similar to those previously detected within long-chain n-alkanes suggesting a preservation through geological time and demonstrating the interest of position-specific isotope analysis for studying the evolution of metabolic pathways.

Carbon isotopes Fatty acids biosynthesis Isotope fractionation Isotopomers NMR

SCI

English

Others

Japan Society for the Promotion of Science (JSPS)["17H06105","18H01326","P17725"] ; Chinese NSF["41973072","42007408"] ; China Postdoctoral Science Foundation[2019M650253]

Geochemistry & Geophysics

Geochemistry & Geophysics

WOS:000886071800004

PERGAMON-ELSEVIER SCIENCE LTD

GEOSCIENCES

2-s2.0-85140450168

Scopus

1.GFZ German Research Centre for Geosciences - Helmholtz Centre Potsdam,Organic Geochemistry,Potsdam,Germany
2.Department of Earth and Planetary Sciences,Tokyo Institute of Technology,Meguro,152-8551 Tokyo,Japan
3.Isotopomics in Chemical Biology (ICB),School of Chemistry & Chemical Engineering,Shaanxi University of Science and Technology,Xi'an,China
4.Department of Ocean Science and Engineering,Southern University of Science and Technology (SUSTech),Shenzhen,518055,China
5.Earth-Life Science Institute,Tokyo Institute of Technology,Meguro,152-8550 Tokyo,Japan
6.Department of Environmental Chemistry and Engineering,Tokyo Institute of Technology,Yokohama,226-8503 Kanagawa,Japan
7.National Institute of Information and Communications Technology,Koganei,184-8795 Tokyo,Japan
8.CNRS,CEISAM UMR 6230,Nantes Université,Nantes,2 rue de la Houssinière, BP 92208,44000,France

Julien，Maxime,Zhao，Yu,Ma，Ran,et al. Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids[J]. ORGANIC GEOCHEMISTRY,2022,174.

Julien，Maxime.,Zhao，Yu.,Ma，Ran.,Zhou，Youping.,Nakagawa，Mayuko.,...&Gilbert，Alexis.(2022).Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids.ORGANIC GEOCHEMISTRY,174.

Julien，Maxime,et al."Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids".ORGANIC GEOCHEMISTRY 174(2022).