Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids
Carbon-13 position-specific isotope analysis of fatty acids from vegetable oils was performed using Nuclear Magnetic Resonance in the present study. The measured C patterns are not totally in accordance with the conventional view of the relative C-depletion of acetogenic lipids and their alternation of C-enriched and C-depleted carbon positions. The results presented here provide a new evaluation of the isotopic fractionation associated with fatty acids biosynthesis. Whereas it is commonly acknowledged that the pyruvate dehydrogenase (PDH) is responsible for the C distribution within fatty acids, data from the present work demonstrate that the conversion of acetyl-CoA to malonyl-CoA catalyzed by acetyl-CoA carboxylase (ACC) needs to be considered while explaining the measured non-stochastic C pattern within fatty acids. These data combined with steady-state calculations give a new description of metabolic steps responsible for the typical C intramolecular distribution of acetogenic lipids. In addition, the non-stochastic pattern measured in these plant fatty acids is similar to those previously detected within long-chain n-alkanes suggesting a preservation through geological time and demonstrating the interest of position-specific isotope analysis for studying the evolution of metabolic pathways.
Japan Society for the Promotion of Science (JSPS)["17H06105","18H01326","P17725"] ; Chinese NSF["41973072","42007408"] ; China Postdoctoral Science Foundation[2019M650253]
|WOS Research Area|
Geochemistry & Geophysics
Geochemistry & Geophysics
|WOS Accession No|
|ESI Research Field|
Cited Times [WOS]:0
|Document Type||Journal Article|
|Department||Department of Ocean Science and Engineering|
1.GFZ German Research Centre for Geosciences - Helmholtz Centre Potsdam,Organic Geochemistry,Potsdam,Germany
2.Department of Earth and Planetary Sciences,Tokyo Institute of Technology,Meguro,152-8551 Tokyo,Japan
3.Isotopomics in Chemical Biology (ICB),School of Chemistry & Chemical Engineering,Shaanxi University of Science and Technology,Xi'an,China
4.Department of Ocean Science and Engineering,Southern University of Science and Technology (SUSTech),Shenzhen,518055,China
5.Earth-Life Science Institute,Tokyo Institute of Technology,Meguro,152-8550 Tokyo,Japan
6.Department of Environmental Chemistry and Engineering,Tokyo Institute of Technology,Yokohama,226-8503 Kanagawa,Japan
7.National Institute of Information and Communications Technology,Koganei,184-8795 Tokyo,Japan
8.CNRS,CEISAM UMR 6230,Nantes Université,Nantes,2 rue de la Houssinière, BP 92208,44000,France
Julien，Maxime,Zhao，Yu,Ma，Ran,et al. Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids[J]. ORGANIC GEOCHEMISTRY,2022,174.
Julien，Maxime.,Zhao，Yu.,Ma，Ran.,Zhou，Youping.,Nakagawa，Mayuko.,...&Gilbert，Alexis.(2022).Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids.ORGANIC GEOCHEMISTRY,174.
Julien，Maxime,et al."Re-evaluation of the 13C isotope fractionation associated with lipids biosynthesis by position-specific isotope analysis of plant fatty acids".ORGANIC GEOCHEMISTRY 174(2022).
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