| Title | Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice |
| Author | Zhou, Tian1; Li, Yuxin2,3; Li, Xiaoyu2,3; Zeng, Fanzhuo2,4; Rao, Yanxia5,6; He, Yang2,3; Wang, Yafei2,3; Liu, Meizhen7; Li, Dali7; Xu, Zhen1; Zhou, Xin2; Du, Siling2,6; Niu, Fugui8  ; Peng, Jiyun9; Mei, Xifan4; Ji, Sheng-Jian8; Shu, Yousheng2,3; Lu, Wei2,3; Guo, Feifan2; Wu, Tianzhun1; Yuan, Ti Fei6; Mao, Ying3; Peng, Bo2,3,10
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| Corresponding Author | Peng, Bo |
| Publication Years | 2022-10-24
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| DOI | |
| Source Title | |
| EISSN | 2041-1723
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| Volume | 13Issue:1 |
| Abstract | Microglia are important immune cells in the central nervous system (CNS) that undergo turnover throughout the lifespan. If microglial debris is not removed in a timely manner, accumulated debris may influence CNS function. Clearance of microglial debris is crucial for CNS homeostasis. However, underlying mechanisms remain obscure. We here investigate how dead microglia are removed. We find that although microglia can phagocytose microglial debris in vitro, the territory-dependent competition hinders the microglia-to-microglial debris engulfment in vivo. In contrast, microglial debris is mainly phagocytosed by astrocytes in the brain, facilitated by C4b opsonization. The engulfed microglial fragments are then degraded in astrocytes via RUBICON-dependent LC3-associated phagocytosis (LAP), a form of noncanonical autophagy. Interference with C4b-mediated engulfment and subsequent LAP disrupt the removal and degradation of microglial debris, respectively. Together, we elucidate the cellular and molecular mechanisms of microglial debris removal in mice, extending the knowledge on the maintenance of CNS homeostasis. |
| URL | [Source Record] |
| Indexed By | |
| Language | English
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| Important Publications | NI Journal Papers
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| SUSTech Authorship | Others
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| Funding Project | National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[32170958]
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| WOS Research Area | Science & Technology - Other Topics
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| WOS Subject | Multidisciplinary Sciences
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| WOS Accession No | WOS:000871563700004
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| Publisher | |
| Scopus EID | 2-s2.0-85140563371
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| Data Source | Scopus
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| Citation statistics |
Cited Times [WOS]:13
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| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/407101 |
| Department | Department of Biology 生命科学学院 |
| Affiliation | 1.Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong,518055,China 2.Department of Neurology,Jinshan Hospital,Institute for Translational Brain Research,State Key Laboratory of Medical Neurobiology,MOE Frontiers Center for Brain Science,Fudan University,Shanghai,201508,China 3.Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai,200040,China 4.Department of Orthopedics,The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou,Liaoning,121012,China 5.Department of Laboratory Animal Science,MOE Frontiers Center for Brain Science,Fudan University,Shanghai,200032,China 6.Shanghai Key Laboratory of Psychotic Disorders,Shanghai Mental Health Center,Shanghai Jiao Tong University School of Medicine,Shanghai,201108,China 7.Shanghai Frontiers Science Center of Genome Editing and Cell Therapy,Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences,East China Normal University,Shanghai,200241,China 8.School of Life Sciences,Department of Biology,Shenzhen Key Laboratory of Gene Regulation and Systems Biology,Brain Research Center,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China 9.Institute of Life Science,Nanchang University,Nanchang,Jiangxi,330031,China 10.Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu,226001,China |
| Recommended Citation GB/T 7714 |
Zhou, Tian,Li, Yuxin,Li, Xiaoyu,et al. Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice[J]. Nature Communications,2022,13(1).
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| APA |
Zhou, Tian.,Li, Yuxin.,Li, Xiaoyu.,Zeng, Fanzhuo.,Rao, Yanxia.,...&Peng, Bo.(2022).Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice.Nature Communications,13(1).
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| MLA |
Zhou, Tian,et al."Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice".Nature Communications 13.1(2022).
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| File Name/Size | DocType | Version | Access | License | ||
| Microglial debris is（11207KB） | Open Access | -- | View | |||
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