| Title | Control of ribosomal RNA synthesis by hematopoietic transcription factors |
| Author | |
| Publication Years | 2022-10-20
|
| DOI | |
| Source Title | |
| ISSN | 1097-2765
|
| EISSN | 1097-4164
|
| Volume | 82Issue:20Pages:3826-3839 |
| Abstract | Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA binding, caused rapid reduction in rRNA transcription due to reduced RNA Pol I occupancy. Our work identifies numerous potential rRNA regulators and provides a template for dissection of TF roles in rRNA transcription. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| Important Publications | NI Journal Papers
|
| SUSTech Authorship | Others
|
| Funding Project | National Heart, Lung, and Blood Institute[R01 HL155144]
; National Institute of General Medical Sciences[R35 GM138035]
|
| WOS Research Area | Biochemistry & Molecular Biology
; Cell Biology
|
| WOS Subject | Biochemistry & Molecular Biology
; Cell Biology
|
| WOS Accession No | WOS:000880784300011
|
| Publisher | |
| ESI Research Field | MOLECULAR BIOLOGY & GENETICS
|
| Scopus EID | 2-s2.0-85140418026
|
| Data Source | Scopus
|
| Citation statistics |
Cited Times [WOS]:3
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/407104 |
| Department | Department of Biochemistry 南方科技大学医学院 |
| Affiliation | 1.Division of Hematology and Oncology,Department of Medicine,University of Pennsylvania Perelman School of Medicine,Philadelphia,PA 19104,United States 2.Institute for Biomedical Informatics,University of Pennsylvania Perelman School of Medicine,Philadelphia,PA 19104,United States 3.College of Arts and Sciences,University of Pennsylvania,Philadelphia,PA 19104,United States 4.Cardiovascular Institute,University of Pennsylvania Perelman School of Medicine,Philadelphia,PA 19104,United States 5.Biochemistry and Molecular Biophysics Graduate Group,University of Pennsylvania Perelman School of Medicine,Philadelphia,PA 19104,United States 6.Beijing Advanced Innovation Center for Genomics (ICG) & Biomedical Pioneering Innovation Center (BIOPIC),Peking University,100871,China 7.Division of Hematology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Shenzhen, China 8.Laboratory of Growth and Development,St Patrick Research Group in Basic Oncology,Cancer Division of the Quebec University Hospital Research Centre (CRCHU de Québec-Université Laval),Canada 9.Laboratory of Growth and Development, St Patrick Research Group in Basic Oncology, Cancer Division of the Quebec University Hospital Research Centre (CRCHU de Québec-Université Laval), Québec, QC G1R 3S3, Canada; Department of Molecular Biology, Medical Biochemistry and Pathology, Laval University, Québec, QC G1V 0A6, Canada 10.Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Genetics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA 11.Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Therapeutic Innovation Center,Baylor College of Medicine,Houston,TX 77030,United States 12.School of Biological Sciences, University of Auckland, Auckland 0623, New Zealand; Digital Life Institute, University of Auckland, Auckland 0632, New Zealand 13.Cancer and Blood Disorders Center,Dana-Farber Cancer Institute and Boston Children's Hospital,Harvard Medical School,Boston,MA 02215,United States 14.Division of Hematology and Oncology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Abramson Family Cancer Research Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA |
| Recommended Citation GB/T 7714 |
Antony，Charles,George，Subin S.,Blum，Justin,et al. Control of ribosomal RNA synthesis by hematopoietic transcription factors[J]. MOLECULAR CELL,2022,82(20):3826-3839.
|
| APA |
Antony，Charles.,George，Subin S..,Blum，Justin.,Somers，Patrick.,Thorsheim，Chelsea L..,...&Paralkar，Vikram R..(2022).Control of ribosomal RNA synthesis by hematopoietic transcription factors.MOLECULAR CELL,82(20),3826-3839.
|
| MLA |
Antony，Charles,et al."Control of ribosomal RNA synthesis by hematopoietic transcription factors".MOLECULAR CELL 82.20(2022):3826-3839.
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| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 2022 Control of ribo（4571KB） | Open Access | -- | View | |||
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