Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis
As an essential modification, O-linked β-N-acetylglucosamine (O-GlcNAc) modulates the functions of many proteins. However, site-specific characterization of O-GlcNAcylated proteins remains challenging. Herein, an innovative material grafted with nitro-oxide (N→O) groups was designed for high affinity enrichment for O-GlcNAc peptides from native proteins. By testing with synthetic O-GlcNAc peptides and standard proteins, the synthesized material exhibited high affinity and selectivity. Based on the material prepared, we developed a workflow for site-specific analysis of O-GlcNAcylated proteins in complex samples. We performed O-GlcNAc proteomics with the PANC-1 cell line, a representative model for pancreatic ductal adenocarcinoma. In total 364 O-GlcNAc peptides from 267 proteins were identified from PANC-1 cells. Among them, 183 proteins were newly found to be O-GlcNAcylated in humans (with 197 O-GlcNAc sites newly reported). The materials and methods can be facilely applied for site-specific O-GlcNAc proteomics in other complex samples.
|WOS Research Area|
Science & Technology - Other Topics ; Materials Science
Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
|WOS Accession No|
Cited Times [WOS]:1
|Document Type||Journal Article|
|Department||Department of Chemistry|
1.Department of Oncology,Lombardi Comprehensive Cancer Center,Georgetown University Medical Center,Washington,20007,United States
2.Catalysis Center for Energy Innovation,University of Delaware,Newark,19716,United States
3.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China
Wu，Ci,Shi，Song,Hou，Chunyan,et al. Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis[J]. ACS Applied Materials & Interfaces,2022.
Wu，Ci,Shi，Song,Hou，Chunyan,Luo，Yang,Byers，Stephen,&Ma，Junfeng.(2022).Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis.ACS Applied Materials & Interfaces.
Wu，Ci,et al."Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis".ACS Applied Materials & Interfaces (2022).
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