Title | Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis |
Author | |
Corresponding Author | Ma,Junfeng |
Publication Years | 2022
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DOI | |
Source Title | |
ISSN | 1944-8244
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EISSN | 1944-8252
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Abstract | As an essential modification, O-linked β-N-acetylglucosamine (O-GlcNAc) modulates the functions of many proteins. However, site-specific characterization of O-GlcNAcylated proteins remains challenging. Herein, an innovative material grafted with nitro-oxide (N→O) groups was designed for high affinity enrichment for O-GlcNAc peptides from native proteins. By testing with synthetic O-GlcNAc peptides and standard proteins, the synthesized material exhibited high affinity and selectivity. Based on the material prepared, we developed a workflow for site-specific analysis of O-GlcNAcylated proteins in complex samples. We performed O-GlcNAc proteomics with the PANC-1 cell line, a representative model for pancreatic ductal adenocarcinoma. In total 364 O-GlcNAc peptides from 267 proteins were identified from PANC-1 cells. Among them, 183 proteins were newly found to be O-GlcNAcylated in humans (with 197 O-GlcNAc sites newly reported). The materials and methods can be facilely applied for site-specific O-GlcNAc proteomics in other complex samples. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | Others
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Funding Project | [NCI-P30 CA051008]
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WOS Research Area | Science & Technology - Other Topics
; Materials Science
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WOS Subject | Nanoscience & Nanotechnology
; Materials Science, Multidisciplinary
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WOS Accession No | WOS:000874100200001
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Publisher | |
Scopus EID | 2-s2.0-85140336300
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Data Source | Scopus
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Citation statistics |
Cited Times [WOS]:2
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/407160 |
Department | Department of Chemistry |
Affiliation | 1.Department of Oncology,Lombardi Comprehensive Cancer Center,Georgetown University Medical Center,Washington,20007,United States 2.Catalysis Center for Energy Innovation,University of Delaware,Newark,19716,United States 3.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China |
Recommended Citation GB/T 7714 |
Wu,Ci,Shi,Song,Hou,Chunyan,et al. Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis[J]. ACS Applied Materials & Interfaces,2022.
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APA |
Wu,Ci,Shi,Song,Hou,Chunyan,Luo,Yang,Byers,Stephen,&Ma,Junfeng.(2022).Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis.ACS Applied Materials & Interfaces.
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MLA |
Wu,Ci,et al."Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis".ACS Applied Materials & Interfaces (2022).
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