Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis

Wu，Ci1; Shi，Song2; Hou，Chunyan1; Luo，Yang3; Byers，Stephen1; Ma，Junfeng1

2022

10.1021/acsami.2c15039

ACS Applied Materials & Interfaces   Impact Factor & Quartile

1944-8244

1944-8252

As an essential modification, O-linked β-N-acetylglucosamine (O-GlcNAc) modulates the functions of many proteins. However, site-specific characterization of O-GlcNAcylated proteins remains challenging. Herein, an innovative material grafted with nitro-oxide (N→O) groups was designed for high affinity enrichment for O-GlcNAc peptides from native proteins. By testing with synthetic O-GlcNAc peptides and standard proteins, the synthesized material exhibited high affinity and selectivity. Based on the material prepared, we developed a workflow for site-specific analysis of O-GlcNAcylated proteins in complex samples. We performed O-GlcNAc proteomics with the PANC-1 cell line, a representative model for pancreatic ductal adenocarcinoma. In total 364 O-GlcNAc peptides from 267 proteins were identified from PANC-1 cells. Among them, 183 proteins were newly found to be O-GlcNAcylated in humans (with 197 O-GlcNAc sites newly reported). The materials and methods can be facilely applied for site-specific O-GlcNAc proteomics in other complex samples.

glycopeptide enrichment glycoproteomics hydrogen bonding interaction nitro-oxide-grafted nanosphere O-GlcNAc O-GlcNAcylation

SCI

English

Others

[NCI-P30 CA051008]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000874100200001

AMER CHEMICAL SOC

2-s2.0-85140336300

Scopus

1.Department of Oncology,Lombardi Comprehensive Cancer Center,Georgetown University Medical Center,Washington,20007,United States
2.Catalysis Center for Energy Innovation,University of Delaware,Newark,19716,United States
3.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China

Wu，Ci,Shi，Song,Hou，Chunyan,et al. Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis[J]. ACS Applied Materials & Interfaces,2022.

Wu，Ci,Shi，Song,Hou，Chunyan,Luo，Yang,Byers，Stephen,&Ma，Junfeng.(2022).Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis.ACS Applied Materials & Interfaces.

Wu，Ci,et al."Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis".ACS Applied Materials & Interfaces (2022).