中文版 | English
Title

Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis

Author
Corresponding AuthorMa,Junfeng
Publication Years
2022
DOI
Source Title
ISSN
1944-8244
EISSN
1944-8252
Abstract
As an essential modification, O-linked β-N-acetylglucosamine (O-GlcNAc) modulates the functions of many proteins. However, site-specific characterization of O-GlcNAcylated proteins remains challenging. Herein, an innovative material grafted with nitro-oxide (N→O) groups was designed for high affinity enrichment for O-GlcNAc peptides from native proteins. By testing with synthetic O-GlcNAc peptides and standard proteins, the synthesized material exhibited high affinity and selectivity. Based on the material prepared, we developed a workflow for site-specific analysis of O-GlcNAcylated proteins in complex samples. We performed O-GlcNAc proteomics with the PANC-1 cell line, a representative model for pancreatic ductal adenocarcinoma. In total 364 O-GlcNAc peptides from 267 proteins were identified from PANC-1 cells. Among them, 183 proteins were newly found to be O-GlcNAcylated in humans (with 197 O-GlcNAc sites newly reported). The materials and methods can be facilely applied for site-specific O-GlcNAc proteomics in other complex samples.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
[NCI-P30 CA051008]
WOS Research Area
Science & Technology - Other Topics ; Materials Science
WOS Subject
Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Accession No
WOS:000874100200001
Publisher
Scopus EID
2-s2.0-85140336300
Data Source
Scopus
Citation statistics
Cited Times [WOS]:2
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/407160
DepartmentDepartment of Chemistry
Affiliation
1.Department of Oncology,Lombardi Comprehensive Cancer Center,Georgetown University Medical Center,Washington,20007,United States
2.Catalysis Center for Energy Innovation,University of Delaware,Newark,19716,United States
3.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China
Recommended Citation
GB/T 7714
Wu,Ci,Shi,Song,Hou,Chunyan,et al. Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis[J]. ACS Applied Materials & Interfaces,2022.
APA
Wu,Ci,Shi,Song,Hou,Chunyan,Luo,Yang,Byers,Stephen,&Ma,Junfeng.(2022).Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis.ACS Applied Materials & Interfaces.
MLA
Wu,Ci,et al."Design and Preparation of Novel Nitro-Oxide-Grafted Nanospheres with Enhanced Hydrogen Bonding Interaction for O-GlcNAc Analysis".ACS Applied Materials & Interfaces (2022).
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