Preparation and properties of porous hydroxyapatite scaffolds with biphase calcium phosphate coating

Jingna，Li1,2; Qingfeng，Zeng3,4; Shuyin，Yu3; Yue，Qin3; Zizheng，Ai5; Xieping，Dong1,6,7,8

2023-06-08

10.12307/2023.074

中国组织工程研究   Impact Factor & Quartile

2095-4344

BACKGROUND: At present, the majority of artificial bone can only be prepared into small-volume filling materials. Large sections of load-bearing bone and large-scale structural bone graft materials still face two major problems: lack of ideal bone defect repair materials and difficulty in material processing and regulating process. OBJECTIVE: To prepare the hydroxyapatite porous bone tissue scaffold with biphase calcium phosphate coating, which is similar to the morphology and structure of human large weight-bearing bone and has certain mechanical properties, excellent biocompatibility and partial degradability and assess its property. METHODS: Two different morphologies of hydroxyapatite (powder and whisker) and β-tricalcium phosphate powder were prepared by hydrothermal synthesis method combined with spray drying technology to evaluate the cytotoxicity of hydroxyapatite powder and β-tricalcium phosphate powder extract. Using 3D printing technology, the mixture of hydroxyapatite powder and whiskers (5%, 10%, and 15%) was prepared into a porous bone embryo scaffold. Different sintering processes were set, and the optimal combination of whisker content and sintering process, such as mechanical properties and porosity, was screened through orthogonal experiments. The hydrodynamics of the optimal combination scaffold was tested. The biphase calcium phosphate coating was coated on the porous scaffold by dipping and pulling method to in vitro assess the degradation performance of β-tricalcium phosphate powder in biphase calcium phosphate coating. RESULTS AND CONCLUSION: (1) MTT assay showed that hydroxyapatite powder and β-tricalcium phosphate powder did not have apparent cytotoxicity. (2) Orthogonal experiment results exhibited that sintering temperature, whisker content, and sintering time were the most important factors affecting the mechanical properties of the scaffold. The optimal parameters of the prepared scaffold were explored: sintering temperature of 1 300 ℃, whisker content of 10%, sintering time of 3 hours. At this time, the compressive strength of the scaffold was 8.13 MPa and the porosity was 64.07%. (3) The simulation of fluid mechanics showed that the way of inflow and outflow of cell nutrient solution perpendicular to the cross-sectional direction of artificial bone was more conducive to the effect of the scaffold in the human body. (4) In the optical mode of the Phenom electron microscope, the pore size of the porous scaffold was 400 μm before impregnation and 250 μm after impregnation. The thickness of the coating was approximately 75 μm. β-Tricalcium phosphate in composite coating exhibited good in vitro degradation properties. (5) These results confirm that the prepared hydroxyapatite/biphase calcium phosphate coating composite scaffold is a kind of bioactive ceramic scaffold with certain high porosity and mechanical properties, excellent biocompatibility and partial degradability.

3D printing biphase calcium phosphate hydroxyapatite whisker mechanical property porous scaffold

English

Corresponding

National Natural Science Foundation of China[81860384];

2-s2.0-85141745680

Scopus

1.Department of Orthopedics,Jiangxi Provincial People’s Hospital,Nanchang,Jiangxi Province,330006,China
2.Graduate School of Jiangxi University of Chinese Medicine,Nanchang,Jiangxi Province,330004,China
3.MSEA International Institute for Materials Genome,Langfang,Hebei Province,065500,China
4.School of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an,Shaanxi Province,710072,China
5.The Fourth Hospital of Nanchang,Nanchang,Jiangxi Province,330104,China
6.Medical College of Nanchang University,Nanchang,Jiangxi Province,330006,China
7.Digital Lab of Orthopeadics,Key Laboratory of Health Commission of Jiangxi Province,Nanchang,Jiangxi Province,330006,China
8.Intelligent and Digital Surgery Innovation Center of SUSTech,Southern University of Science and Technology Hospital,Shenzhen,Guangdong Province,518000,China

Jingna，Li,Qingfeng，Zeng,Shuyin，Yu,et al. Preparation and properties of porous hydroxyapatite scaffolds with biphase calcium phosphate coating[J]. 中国组织工程研究,2023,27(16):2493-2500.

Jingna，Li,Qingfeng，Zeng,Shuyin，Yu,Yue，Qin,Zizheng，Ai,&Xieping，Dong.(2023).Preparation and properties of porous hydroxyapatite scaffolds with biphase calcium phosphate coating.中国组织工程研究,27(16),2493-2500.

Jingna，Li,et al."Preparation and properties of porous hydroxyapatite scaffolds with biphase calcium phosphate coating".中国组织工程研究 27.16(2023):2493-2500.