Recent progress in CRISPR-based microfluidic assays and applications
|Corresponding Author||Jiang，Xingyu; Kong，Jilie; Fang，Xueen|
In the past few years, CRISPR-based (Clustered regularly interspaced short palindromic repeats-based) detection techniques have arisen in the field of bioanalysis, molecular biology and clinical diagnosis. However, there are still some limitations, such as high cost, high technical requirements, complexity, cross contamination, lack of quantification strategies, etc. Microfluidic platforms, as emerging technologies, have the potential to take CRISPR-based technology to the new level in the future. In this review, we illustrate the recent progress as well as our work and collaborative work in CRISPR-based microfluidic detection assays based on the categories of microfluidic chips, including paper-based microfluidics, centrifugal microfluidics, digital microfluidics, electrochemical microfluidics and wearable microfluidics, mainly focusing on how microfluidic platforms improve CRISPR-based detection method in the aspect of visualization, detection throughput, anti-contamination, simple operation, sensitivity, specificity and quantification, etc. And we outlook the future trends of CRISPR-based microfluidic detection methods in both innovation of CRISPR-based and design of microfluidic platforms.
China Postdoctoral Science Foundation[2021M700806];National Natural Science Foundation of China;National Natural Science Foundation of China;National Natural Science Foundation of China;National Natural Science Foundation of China;
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Cited Times [WOS]:0
|Document Type||Journal Article|
|Department||Department of Biomedical Engineering|
1.Department of Chemistry and Institutes of Biomedical Sciences,Fudan University,Shanghai,200433,China
2.Shenzhen Key Laboratory of Smart Healthcare Engineering,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
|Corresponding Author Affilication||Department of Biomedical Engineering|
Li，Pintao,Xiong，Huiwen,Yang，Bin,et al. Recent progress in CRISPR-based microfluidic assays and applications[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2022,157.
Li，Pintao,Xiong，Huiwen,Yang，Bin,Jiang，Xingyu,Kong，Jilie,&Fang，Xueen.(2022).Recent progress in CRISPR-based microfluidic assays and applications.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,157.
Li，Pintao,et al."Recent progress in CRISPR-based microfluidic assays and applications".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 157(2022).
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