Recent progress in CRISPR-based microfluidic assays and applications

Li，Pintao1; Xiong，Huiwen1; Yang，Bin1; Jiang，Xingyu2; Kong，Jilie1; Fang，Xueen1

2022-12-01

10.1016/j.trac.2022.116812

TRAC-TRENDS IN ANALYTICAL CHEMISTRY   Impact Factor & Quartile

0165-9936

1879-3142

In the past few years, CRISPR-based (Clustered regularly interspaced short palindromic repeats-based) detection techniques have arisen in the field of bioanalysis, molecular biology and clinical diagnosis. However, there are still some limitations, such as high cost, high technical requirements, complexity, cross contamination, lack of quantification strategies, etc. Microfluidic platforms, as emerging technologies, have the potential to take CRISPR-based technology to the new level in the future. In this review, we illustrate the recent progress as well as our work and collaborative work in CRISPR-based microfluidic detection assays based on the categories of microfluidic chips, including paper-based microfluidics, centrifugal microfluidics, digital microfluidics, electrochemical microfluidics and wearable microfluidics, mainly focusing on how microfluidic platforms improve CRISPR-based detection method in the aspect of visualization, detection throughput, anti-contamination, simple operation, sensitivity, specificity and quantification, etc. And we outlook the future trends of CRISPR-based microfluidic detection methods in both innovation of CRISPR-based and design of microfluidic platforms.

Centrifugal CRISPR Droplet-based Electrochemical Microarray Microfluidic Paper-based Wearable

SCI

English

Corresponding

China Postdoctoral Science Foundation[2021M700806];National Natural Science Foundation of China[21974028];National Natural Science Foundation of China[22127806];National Natural Science Foundation of China[22174022];National Natural Science Foundation of China[22174024];

Chemistry

Chemistry, Analytical

WOS:000886660800005

ELSEVIER SCI LTD

CHEMISTRY

2-s2.0-85141477162

Scopus

1.Department of Chemistry and Institutes of Biomedical Sciences,Fudan University,Shanghai,200433,China
2.Shenzhen Key Laboratory of Smart Healthcare Engineering,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Li，Pintao,Xiong，Huiwen,Yang，Bin,et al. Recent progress in CRISPR-based microfluidic assays and applications[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2022,157.

Li，Pintao,Xiong，Huiwen,Yang，Bin,Jiang，Xingyu,Kong，Jilie,&Fang，Xueen.(2022).Recent progress in CRISPR-based microfluidic assays and applications.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,157.

Li，Pintao,et al."Recent progress in CRISPR-based microfluidic assays and applications".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 157(2022).