中文版 | English
Title

Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline

Author
Corresponding AuthorTse,Yu Chung
Publication Years
2022-11-03
DOI
Source Title
EISSN
2058-7716
Volume8Issue:1
Abstract

Apoptosis is one of the major forms of programmed cell death, and it serves vital biological functions in multicellular animal and plant cells. The core mechanism of apoptosis is highly conserved in metazoans, where the translocation of CED-4/Apaf-1 from mitochondria to the nuclear membrane is required to initiate and execute apoptosis. However, the underlying molecular mechanisms of this translocation are poorly understood. In this study, we showed that SAO-1 binds DLC-1 and prevents its degradation to promote apoptosis in C. elegans germ cells. We demonstrated that SAO-1 and DLC-1 regulate CED-4/Apaf-1 nuclear membrane accumulation during apoptosis. Isothermal titration calorimetry-based assay and high-resolution crystal structure analysis further revealed that SAO-1 interacted with DLC-1 to form a 2:4 complex: each of the two β-sheets in the SAO-1 peptide interacted with two DLC-1 dimers. Point mutations at the SAO-1-DLC-1 binding interface significantly inhibited apoptotic corpse formation and CED-4 nuclear membrane accumulation within C. elegans germ cells. In conclusion, our study provides a new perspective on the regulation of CED-4-mediated apoptosis.

URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
Natural Science Foundation of Guangdong Province[2020A1515010742] ; National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[31671409] ; National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[31870757] ; National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[32170697]
WOS Research Area
Cell Biology
WOS Subject
Cell Biology
WOS Accession No
WOS:000878148600002
Publisher
Scopus EID
2-s2.0-85141083545
Data Source
Scopus
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/411782
DepartmentDepartment of Biology
生命科学学院
公共分析测试中心
南方科技大学医学院
Affiliation
1.School of Life Science and Technology,Harbin Institute of Technology,Harbin,150001,China
2.School of Life Sciences,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
3.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,Shenzhen,518055,China
4.School of Biological Sciences,Faculty of Science,The University of Hong Kong,Hong Kong
5.Department of Biology,State Key Laboratory of Environmental and Biological Analysis,Hong Kong Baptist University,Hong Kong
6.Core Research Facilities,Southern University of Science and Technology,Shenzhen,518055,China
First Author AffilicationDepartment of Biology;  School of Life Sciences;  School of Medicine
Corresponding Author AffilicationSchool of Medicine;  Public Testing and Analysis Center
Recommended Citation
GB/T 7714
Zhang,Dandan,Yang,Haibin,Jiang,Ling,et al. Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline[J]. Cell Death Discovery,2022,8(1).
APA
Zhang,Dandan.,Yang,Haibin.,Jiang,Ling.,Zhao,Chan.,Wang,Mengjun.,...&Tse,Yu Chung.(2022).Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline.Cell Death Discovery,8(1).
MLA
Zhang,Dandan,et al."Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline".Cell Death Discovery 8.1(2022).
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