Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry
Mechanosensing is an integral part of many physiological processes including stem cell differentiation, fibrosis, and cancer progression. Two major mechanosensing systems-focal adhesions and mechanosensitive ion channels-can convert mechanical features of the microenvironment into biochemical signals. We report here unexpectedly that the mechanosensitive calcium-permeable channel Piezo1, previously perceived to be diffusive on plasma membranes, binds to matrix adhesions in a force-dependent manner, promoting cell spreading, adhesion dynamics, and calcium entry in normal but not in most cancer cells tested except some glioblastoma lines. A linker domain in Piezo1 is needed for binding to adhesions, and overexpression of the domain blocks Piezo1 binding to adhesions, decreasing adhesion size and cell spread area. Thus, we suggest that Piezo1 is a previously unidentified component of focal adhesions in nontransformed cells that catalyzes adhesion maturation and growth through force-dependent calcium signaling, but this function is absent in most cancer cells.
Cited Times [WOS]:2
|Document Type||Journal Article|
|Department||Department of Biomedical Engineering|
1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Guangdong Provincial Key Laboratory of Advanced Biomaterials,Southern University of Science and Technology,Shenzhen,518055,China
3.Mechanobiology Institute,National University of Singapore,117411,Singapore
4.Center for Biosystems Science and Engineering,Indian Institute of Science,India
5.Victor Chang Cardiac Research Institute,Australia
6.Ecole Normale Supérieure Paris-Saclay Gif-sur-Yvette,France
7.Team Ca2+ Signaling and Microbial Infections,Institute for Integrative Biology of the Cell-CNRS UMR9198-Inserm U1280,91190 Gif-sur-Yvette,Avenue de la Terrasse,France
8.Department of Biological Sciences,National University of Singapore,117558,Singapore
9.Molecular MechanoMedicine Program,Department of Biochemistry and Molecular Biology,University of Texas Medical Branch,Galveston,United States
|First Author Affilication||Department of Biomedical Engineering; Southern University of Science and Technology|
|First Author's First Affilication||Department of Biomedical Engineering|
Yao，Mingxi,Tijore，Ajay,Cheng，Delfine,et al. Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry[J]. Science advances,2022,8(45).
Yao，Mingxi.,Tijore，Ajay.,Cheng，Delfine.,Li，Jinyuan Vero.,Hariharan，Anushya.,...&Sheetz，Michael.(2022).Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry.Science advances,8(45).
Yao，Mingxi,et al."Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry".Science advances 8.45(2022).
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