Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles

Di，Xiangjun1; Wang，Dejiang1; Su，Qian Peter2; Liu，Yongtao1; Liao，Jiayan1; Maddahfar，Mahnaz1; Zhou，Jiajia1; Jin，Dayong1,3

2022-11-08

10.1073/pnas.2207402119

Proceedings of the National Academy of Sciences of the United States of America   Impact Factor & Quartile

1091-6490

The intracellular metabolism of organelles, like lysosomes and mitochondria, is highly coordinated spatiotemporally and functionally. The activities of lysosomal enzymes significantly rely on the cytoplasmic temperature, and heat is constantly released by mitochondria as the byproduct of adenosine triphosphate (ATP) generation during active metabolism. Here, we developed temperature-sensitive LysoDots and MitoDots to monitor the in situ thermal dynamics of lysosomes and mitochondria. The design is based on upconversion nanoparticles (UCNPs) with high-density surface modifications to achieve the exceptionally high sensitivity of 2.7% K-1 and low uncertainty of 0.8 K for nanothermometry to be used in living cells. We show the measurement is independent of the ion concentrations and pH values. With Ca2+ ion shock, the temperatures of both lysosomes and mitochondria increased by ∼2 to 4 °C. Intriguingly, with chloroquine (CQ) treatment, the lysosomal temperature was observed to decrease by up to ∼3 °C, while mitochondria remained relatively stable. Lastly, with oxidative phosphorylation inhibitor treatment, we observed an ∼3 to 7 °C temperature increase and a thermal transition from mitochondria to lysosomes. These observations indicate different metabolic pathways and thermal transitions between lysosomes and mitochondria inside HeLa cells. The nanothermometry probes provide a powerful tool for multimodality functional imaging of subcellular organelles and interactions with high spatial, temporal, and thermal dynamics resolutions.

lysosome mitochondria nanothermometry upconversion nanoparticles (UCNPs)

English

Others

2-s2.0-85141153869

Scopus

1.Institute for Biomedical Materials and Devices,Faculty of Science,University of Technology Sydney,Sydney,NSW 2007,Australia
2.School of Biomedical Engineering,Faculty of Engineering and Information Technology,University of Technology Sydney,Sydney,NSW 2007,Australia
3.UTS-SUStech Joint Research Centre for Biomedical Materials and Devices,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Di，Xiangjun,Wang，Dejiang,Su，Qian Peter,et al. Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles[J]. Proceedings of the National Academy of Sciences of the United States of America,2022,119(45).

Di，Xiangjun.,Wang，Dejiang.,Su，Qian Peter.,Liu，Yongtao.,Liao，Jiayan.,...&Jin，Dayong.(2022).Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles.Proceedings of the National Academy of Sciences of the United States of America,119(45).

Di，Xiangjun,et al."Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles".Proceedings of the National Academy of Sciences of the United States of America 119.45(2022).