Exclusive Enteral Nutrition Exerts Anti-Inflammatory Effects through Modulating Microbiota, Bile Acid Metabolism, and Immune Activities

Xiao，Fangfei1,2; Gao，Xuefeng3,4; Hu，Hui1; Le，Jun1; Chen，Yongheng5; Shu，Xingsheng5; Liang，Ziwei6; Xu，Yang6; Wang，Yizhong1,2; Zhang，Ting1,2

2022-11-01

10.3390/nu14214463

Nutrients   Impact Factor & Quartile

2072-6643

Exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn’s disease (CD). This study aims to depict EEN’s modification of bile acid (BA) metabolism in pediatric CD and explores the effect of the EEN-enriched BA in inhibiting the inflammatory response. The twelve enrolled pediatric CD patients showed BA dysmetabolism, represented by decreased levels of fecal secondary and unconjugated BAs as determined by UPLC–TQMS, which were accompanied by gut microbiota dysbiosis and reduced BA-metabolizing bacteria including Eubacterium and Ruminococcus genera, assessed by shotgun metagenomic sequencing. EEN treatment induced remission in these patients at eight weeks, and nine patients remained in stable remission for longer than 48 weeks. EEN improved BA dysmetabolism, with some enriched BAs, including hyocholic acid (HCA), α-muricholic acid (αMCA), strongly associated with decreased severity of CD symptoms. These BAs were significantly correlated with the increased abundance of certain bacteria, including Clostridium innocuum and Hungatella hathewayi, which express 3β-hydroxysteroid dehydrogenase and 5β-reductase. HCA could suppress TNF-α production by CD4+ T cells in the peripheral blood mononuclear cells (PBMCs) of CD patients. Moreover, intraperitoneal injection of HCA could attenuate dextran sulfate sodium (DSS)-induced mouse colitis. Our data suggests that BA modification may contribute to the EEN-induced remission of pediatric CD.

bile acid Crohn’s disease EEN gut microbiota hyocholic acid

SCI

English

Others

Science and Technology Commission of Shanghai Municipality[19140904300];National Natural Science Foundation of China[81870373];National Natural Science Foundation of China[81900474];Shenzhen Key Laboratory Fund[ZDSYS20200811143757022];

Nutrition & Dietetics

Nutrition & Dietetics

WOS:000881425500001

MDPI

2-s2.0-85141774576

Scopus

1.Department of Gastroenterology,Hepatology and Nutrition,Shanghai Children’s Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,200062,China
2.Institute of Pediatric Infection,Immunity and Critical Care Medicine,Shanghai Children’s Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,200062,China
3.Central Laboratory,Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies,International Cancer Center,Shenzhen University General Hospital,Shenzhen,518000,China
4.Department of Gastroenterology and Hepatology,Shenzhen University General Hospital,Shenzhen,518000,China
5.Department of Physiology,School of Basic Medical Sciences,Shenzhen University Health Science Center,Shenzhen,518000,China
6.School of Medicine,Southern University of Science and Technology,Shenzhen,518100,China

Xiao，Fangfei,Gao，Xuefeng,Hu，Hui,et al. Exclusive Enteral Nutrition Exerts Anti-Inflammatory Effects through Modulating Microbiota, Bile Acid Metabolism, and Immune Activities[J]. Nutrients,2022,14(21).

Xiao，Fangfei.,Gao，Xuefeng.,Hu，Hui.,Le，Jun.,Chen，Yongheng.,...&Zhang，Ting.(2022).Exclusive Enteral Nutrition Exerts Anti-Inflammatory Effects through Modulating Microbiota, Bile Acid Metabolism, and Immune Activities.Nutrients,14(21).

Xiao，Fangfei,et al."Exclusive Enteral Nutrition Exerts Anti-Inflammatory Effects through Modulating Microbiota, Bile Acid Metabolism, and Immune Activities".Nutrients 14.21(2022).