In Vivo Imaging of Exosomes Labeled with NIR-II Polymer Dots in Liver-Injured Mice

Ma，Ning1; Liu，Ye2; Chen，Dandan2; Wu，Changfeng2; Meng，Zihui1

2022

10.1021/acs.biomac.2c01005

BIOMACROMOLECULES   Impact Factor & Quartile

1525-7797

1526-4602

Mesenchymal stem cell-derived exosomes (MSC-Exos) are emerging as a promising platform for treating various intractable diseases and organ injuries. Monitoring their migration, homing, and therapeutic capability in vivo is essential to develop exosome-based theranostics. Here, we designed fluorescent semiconductor polymer dots (Pdots) in the second near-infrared window (NIR-II) for bright labeling and tracking of MSC-Exos. Glucose-coated Pdots (Pdots-Glu) were able to label MSC-Exos without changing their biological properties. The NIR-II fluorescent Pdots allow for high labeling brightness and long-term in vivo tracking of MSC-Exos. We investigated the biodistributions and therapeutic functions of these labeled MSC-Exos in liver-resected mice. In vivo and ex vivo imaging demonstrated that the Pdot-labeled MSC-Exos injected via the tail vein mainly accumulated in the residual liver tissue. In terms of the therapeutic effect, MSC-Exos may accelerate postoperative liver function recovery by inhibiting inflammatory responses, promoting cell proliferation, and resisting apoptosis. Our results indicated that MSC-Exos therapeutic systems hold promising applications in liver regenerative medicine.

SCI

English

Others

[20180201055YY]

Biochemistry & Molecular Biology ; Chemistry ; Polymer Science

Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science

WOS:000878942500001

AMER CHEMICAL SOC

BIOLOGY & BIOCHEMISTRY

2-s2.0-85141644254

Scopus

1.Department of Hepatobiliary-Pancreatic Surgery,China-Japan Union Hospital,Jilin University,Changchun,Jilin,130000,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Ma，Ning,Liu，Ye,Chen，Dandan,et al. In Vivo Imaging of Exosomes Labeled with NIR-II Polymer Dots in Liver-Injured Mice[J]. BIOMACROMOLECULES,2022.

Ma，Ning,Liu，Ye,Chen，Dandan,Wu，Changfeng,&Meng，Zihui.(2022).In Vivo Imaging of Exosomes Labeled with NIR-II Polymer Dots in Liver-Injured Mice.BIOMACROMOLECULES.

Ma，Ning,et al."In Vivo Imaging of Exosomes Labeled with NIR-II Polymer Dots in Liver-Injured Mice".BIOMACROMOLECULES (2022).