中文版 | English
Title

PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3

Author
Corresponding AuthorChunhui Hou; Fuliang Du
Publication Years
2022-06-30
Source Title
EISSN
1943-8141/AJTR0143899
Volume14Issue:6Pages:4295-4309
Abstract

Embryonic stem cell (ESC) research is critical to the scientific community, as their application in regenerative medicine can be widely beneficial. ESCs eventually withdraw from their self-renewal program and subsequently differentiate into specific cell lineages; however, the mechanisms regulating these processes remain unclear. PKC inhibition using 3-[1-[3-(dimethylamino) propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione (PKCi) is responsible for the derivation and maintenance of human, rat, and mouse ESCs, but the mechanism by which PKCi maintains stem cell self-renewal is poorly understood. By studying the PKCi stem cell (PKCi-mESC) transcriptome and epigenetic modification, we found the transcriptome of PKCi-mESC differed from 2i stem cells (2i-mESC), with 2010 up-regulated genes and 1784 down-regulated genes. Among them, genes related to core transcription factors, naïve-specific markers, and pluripotency are differentially expressed between the two stem cell lines. We analyzed epigenetic modification of PKCi-mESC and found the distribution of H3K27me3 signal was significantly reduced at transcription start sites (TSSs) throughout the genome and at differentially expressed genes (DEGs). Likewise, the H3K9me3 signal at TSSs throughout the genome was significantly reduced in PKCi-mESC, but the distribution on DEGs is reversed. Kdm4d and Kdm6a knockdown by RNA interference (RNAi) significantly altered the expression of genes related to self-renewal in PKCi-mESC. In conclusion, we revealed PKCi-mESC and 2i-mESC differentially express numerous genes, including stem cell-related genes. Furthermore, PKCi-mESC regulated gene expression through H3K27me3 and H3K9me3 modification, which maintained stem cell self-renewal capacity.

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Language
English
SUSTech Authorship
Corresponding
Data Source
人工提交
PDF urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274548/
Publication Status
在线出版
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/412003
DepartmentDepartment of Biology
生命科学学院
Affiliation
1.Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University Nanjing 210046, China.
2.Harbin Institute of Technology Harbin 150001, Heilongjiang, China.
3.Shenzhen Key Laboratory of Gene Regulation and Systems Biology, Department of Biology, School of Life Sciences, Southern University of Science and Technology Shenzhen 518055, Guangdong, China.
Corresponding Author AffilicationDepartment of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Jialei Sun,Na He,Weiguo Wang,et al. PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3[J]. Am J Transl Res,2022,14(6):4295-4309.
APA
Jialei Sun,Na He,Weiguo Wang,Yujian Dai,Chunhui Hou,&Fuliang Du.(2022).PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3.Am J Transl Res,14(6),4295-4309.
MLA
Jialei Sun,et al."PKC inhibitors regulate stem cell self-renewal by regulating H3K27me3 and H3K9me3".Am J Transl Res 14.6(2022):4295-4309.
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