Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma

Guan, Jiezhong1; Liu, Xinyu1; Wang, Kang1; Jia, Yiqun2; Yang, Bo1

2022-10-25

10.1515/med-2022-0575

Open Medicine   Impact Factor & Quartile

2391-5463

Head and neck squamous cell carcinoma (HNSCC) is one of the most aggressive malignancies that have a poor prognosis. Necroptosis has been demonstrated in recent years to be a form of inflammatory cell death occurring in multicellular organism, which plays complex roles in cancer. However, the expression of necroptosis-related miRNAs and genes in HNSCC and their correlations with prognosis remain unclear. In this study, R software was used to screen differentially expressed miRNAs downloaded from The Cancer Genome Atlas. A prognostic model containing six necroptosis-related miRNAs (miR-141-3p, miR-148a-3p, miR-331-3p, miR-543, miR-425-5p, and miR-7-5p) was generated, whose risk score was validated as an independent prognostic factor for HNSCC. Target genes of the key miRNAs were obtained from TargetScan, miRDB, and miRTarBase, and 193 genes in the intersection of the three databases were defined as consensus genes. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses indicated that the composition of the tumor microenvironment as well as specific pathways may be closely related to necroptosis in HNSCC. Nine key genes were also obtained by the MCODE and cytoHubba plug-ins of Cytoscape: PIK3CD, NRAS, PTK2, IRS2, IRS1, PARP1, KLF4, SMAD2, and DNMT1. A prognostic model formed by the key gene was also established, which can efficiently predict the overall survival of HNSCC patients. In conclusion, necroptosis-related miRNAs and genes play important roles in tumor development and metastasis and can be used to predict the prognosis of HNSCC.

head and neck cancer necroptosis prognostic markers bioinformatics microRNA

SCI

English

Others

National Natural Science Foundation of China[82001109] ; Fundamental Research Funds for the CentralUniversities[20ykpy80] ; OpenFundingofGuangdong Provincial Key Laboratory of Stomatology["KF2020120102","KF2021120103"] ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation ("Climbing Program" Special Funds)[pdjh2023b0017] ; Undergraduate Training Program for Innovation and Entrepreneurship of Sun Yat-sen University["202210786","202210765","202210763"]

General & Internal Medicine

Medicine, General & Internal

WOS:000871662600001

DE GRUYTER POLAND SP Z O O

Web of Science

1.Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Dept Prosthodont,Guangdong Prov Key Lab Stomatol, Lingyuan West Rd, Guangzhou, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Clin Med Coll 2, Shenzhen Peoples Hosp, Stomatol Ctr,Affiliated Hosp 1,Jinan Univ, Shenzhen, Guangdong, Peoples R China

Guan, Jiezhong,Liu, Xinyu,Wang, Kang,et al. Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma[J]. Open Medicine,2022,17(1).

Guan, Jiezhong,Liu, Xinyu,Wang, Kang,Jia, Yiqun,&Yang, Bo.(2022).Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma.Open Medicine,17(1).

Guan, Jiezhong,et al."Identification of a novel necroptosis-associated miRNA signature for predicting the prognosis in head and neck squamous cell carcinoma".Open Medicine 17.1(2022).