Title | NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy |
Author | |
Corresponding Author | Zhu, Yuanfan; Li, Nini |
Publication Years | 2022-10-01
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DOI | |
Source Title | |
ISSN | 2305-5839
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EISSN | 2305-5847
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Abstract | Background: Intrauterine Listeria monocytogenes (L. monocytogenes) infections pose a major threat during pregnancy via affecting placental immune responses. However, the underlying mechanisms of placental defense against this pathogen remain ill-defined. Therefore, this study aims to investigate the function and the mechanism of inflammasomes on against L. monocytogenes infection during pregnancy.Methods: A listeriosis murine model and cell culture system was used to investigate the role of trophoblastic nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 (NLRP3) in orchestrating innate immune responses to L. monocytogenes infection. Caspase-1 activity was determined using a caspase-1 activity colorimetric kit. NLRP3 and apoptosis-associated speck-like protein containing a CARD (ASC) in placental tissue was detected by immunohistochemistry. NLRP3 in HTR-8/SVneo cells was also detected by immunofluorescence. The expression of interleukin 113 (IL-113), NLRP3, ASC, and caspase-1 was detected by Western blot. We characterized the NLRP3 inflammasome in trophoblast cells according to whether L. monocytogenes infection increased the activation of caspase-1 and the release of IL-113. For human or mouse IL-113 in the culture supernatants and mouse tissue lysates were analyzed using ELISA Kits.Results: Trophoblast cells constitutively expressed the components of the NLRP3 inflammasome. In vitro, L. monocytogenes triggers NLRP3 inflammasome activation in trophoblast cells by inducing caspase-1 activation, increasing the NLRP3 protein levels, IL-113 maturation and secretion in HTR-8/SVneo cells. In vivo, L. monocytogenes induces fetal resorption and IL-113 processing in pregnant mice. In addition, NLRP3-deficient mice were more prone to fetal loss than their wild-type counterparts following infection with L. monocytogenes at a lower infective doseConclusions: We conclude that trophoblast cells respond to L. monocytogenes infection through the NLRP3 receptor, resulting in inflammasome activation and IL-113 production, which prevents listeriosis during pregnancy. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | Corresponding
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Funding Project | [2019A1515011068]
; [JCYJ20210324114009026]
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WOS Research Area | Oncology
; Research & Experimental Medicine
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WOS Subject | Oncology
; Medicine, Research & Experimental
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WOS Accession No | WOS:000887090000001
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Publisher | |
Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:0
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/412174 |
Department | School of Medicine 南方科技大学第二附属医院 |
Affiliation | 1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Obstet & Gynecol, Shenzhen, Peoples R China 2.Jilin Univ, Coll Vet Med, Key Lab Zoonosis, Minist Educ, Changchun, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China 4.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Emergency Dept, Shenzhen, Peoples R China 5.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Cytotherapy Lab, Shenzhen, Peoples R China 6.Jinan Univ, Obstet & Gynecol, Shenzhen Baoan Maternal & Child Hlth Hosp, Shenzhen, Peoples R China 7.First Affiliated Hosp Jinan Univ, Obstet & Gynecol, Guangzhou, Peoples R China 8.Southern Univ Sci & Technol, Affiliated Hosp 2, Biotherapy Res Ctr, Sch Med, Shenzhen, Peoples R China 9.Shenzhen Third Peoples Hosp, Biotherapy Res Ctr, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China |
Corresponding Author Affilication | School of Medicine; The Third People's Hospital of Shenzhen |
Recommended Citation GB/T 7714 |
Gao, Yu,Zhou, Min,Zhang, Wen,et al. NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy[J]. Annals of Translational Medicine,2022.
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APA |
Gao, Yu.,Zhou, Min.,Zhang, Wen.,Jiang, Jinxing.,Ouyang, Zhibin.,...&Li, Nini.(2022).NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy.Annals of Translational Medicine.
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MLA |
Gao, Yu,et al."NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy".Annals of Translational Medicine (2022).
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