中文版 | English
Title

Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub

Author
Corresponding AuthorWei,Zhao
Joint first authorWei,Tan; Sihua,Cheng; Yingying,Li
Publication Years
2022-11-23
DOI
Source Title
EISSN
2041-1723
Volume13Issue:1
Abstract
["The polarization of distinct scaffold-signaling hubs at opposite cell poles constitutes the basis of asymmetric cell division. Here, the authors show that phase separation serves as a general mechanism to regulate the assembly and dynamics of a new-pole scaffold-signaling hub.","Asymmetric cell division (ACD) produces morphologically and behaviorally distinct cells and is the primary way to generate cell diversity. In the model bacterium Caulobacter crescentus, the polarization of distinct scaffold-signaling hubs at the swarmer and stalked cell poles constitutes the basis of ACD. However, mechanisms involved in the formation of these hubs remain elusive. Here, we show that a swarmer-cell-pole scaffold, PodJ, forms biomolecular condensates both in vitro and in living cells via phase separation. The coiled-coil 4-6 and the intrinsically disordered regions are the primary domains that contribute to biomolecular condensate generation and signaling protein recruitment in PodJ. Moreover, a negative regulation of PodJ phase separation by the stalked-cell-pole scaffold protein SpmX is revealed. SpmX impedes PodJ cell-pole accumulation and affects its recruitment ability. Together, by modulating the assembly and dynamics of scaffold-signaling hubs, phase separation may serve as a general biophysical mechanism that underlies the regulation of ACD in bacteria and other organisms."]
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
National Key R&D Program of China[2019YFA0904003] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB38020300] ; Guangdong Basic and Applied Basic Research Foundation["2021A1515012511","2021A1515110923"] ; National Natural Science Foundation of China[32101176]
WOS Research Area
Science & Technology - Other Topics
WOS Subject
Multidisciplinary Sciences
WOS Accession No
WOS:000887967800014
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/412203
DepartmentDepartment of Biology
生命科学学院
Affiliation
1.CASKeyLaboratoryofQuantitativeEngineeringBiology,ShenzhenInstituteofSyntheticBiology,ShenzhenInstitutesofAdvancedTechnology,Chinese AcademyofSciences,Shenzhen518055,China.
2.DepartmentofPharmacy,SchoolofLifeSciences,HenanUniversity,Kaifeng475004,China.
3.CASKey LaboratoryofSyntheticBiology,CASCenterforExcellenceinMolecularPlantSciences,ShanghaiInstituteofPlantPhysiologyandEcology,Chinese AcademyofSciences,Shanghai200032,China.
4.UniversityofChineseAcademyofSciences,Beijing100049,China.
5.DepartmentofMaterialsScienceand Engineering,SchoolofEngineering,SouthernUniversityofScienceandTechnology,Shenzhen518055,China.
6.Department of Biology, School of Life Sciences, Southern University of Science and Technology,Shenzhen 518055, China.
7.State Key Lab of Genetic Engineering & Institutes of Biomedical Sciences,Department of Microbiology and Microbial Engineering, School of Life Sciences,Fudan University,Shanghai200433,China.
8.Departmentof Chemistry,UniversityofPittsburgh,Pittsburgh,PA15260,USA.
Recommended Citation
GB/T 7714
Wei,Tan,Sihua,Cheng,Yingying,Li,et al. Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub[J]. NATURE COMMUNICATIONS,2022,13(1).
APA
Wei,Tan.,Sihua,Cheng.,Yingying,Li.,Xiao-Yang,Li.,Ning,Lu.,...&Wei,Zhao.(2022).Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub.NATURE COMMUNICATIONS,13(1).
MLA
Wei,Tan,et al."Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub".NATURE COMMUNICATIONS 13.1(2022).
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