Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub

Wei,Tan1; Sihua,Cheng1; Yingying,Li1; Xiao-Yang,Li1,2; Ning,Lu1; Jingxian,Sun1; Guiyue,Tang1; Yujiao,Yang3,4; Kezhu,Cai1,5; Xuefei,Li1; Xijun,Ou6; Xiang,Gao1; Guo-Ping,Zhao1,3,7; W. Seth,Childers8; Wei,Zhao1

2022-11-23

10.1038/s41467-022-35000-2

NATURE COMMUNICATIONS   Impact Factor & Quartile

2041-1723

["The polarization of distinct scaffold-signaling hubs at opposite cell poles constitutes the basis of asymmetric cell division. Here, the authors show that phase separation serves as a general mechanism to regulate the assembly and dynamics of a new-pole scaffold-signaling hub.","Asymmetric cell division (ACD) produces morphologically and behaviorally distinct cells and is the primary way to generate cell diversity. In the model bacterium Caulobacter crescentus, the polarization of distinct scaffold-signaling hubs at the swarmer and stalked cell poles constitutes the basis of ACD. However, mechanisms involved in the formation of these hubs remain elusive. Here, we show that a swarmer-cell-pole scaffold, PodJ, forms biomolecular condensates both in vitro and in living cells via phase separation. The coiled-coil 4-6 and the intrinsically disordered regions are the primary domains that contribute to biomolecular condensate generation and signaling protein recruitment in PodJ. Moreover, a negative regulation of PodJ phase separation by the stalked-cell-pole scaffold protein SpmX is revealed. SpmX impedes PodJ cell-pole accumulation and affects its recruitment ability. Together, by modulating the assembly and dynamics of scaffold-signaling hubs, phase separation may serve as a general biophysical mechanism that underlies the regulation of ACD in bacteria and other organisms."]

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National Key R&D Program of China[2019YFA0904003] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB38020300] ; Guangdong Basic and Applied Basic Research Foundation["2021A1515012511","2021A1515110923"] ; National Natural Science Foundation of China[32101176]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000887967800014

NATURE PORTFOLIO

Web of Science

1.CASKeyLaboratoryofQuantitativeEngineeringBiology,ShenzhenInstituteofSyntheticBiology,ShenzhenInstitutesofAdvancedTechnology,Chinese AcademyofSciences,Shenzhen518055,China.
2.DepartmentofPharmacy,SchoolofLifeSciences,HenanUniversity,Kaifeng475004,China.
3.CASKey LaboratoryofSyntheticBiology,CASCenterforExcellenceinMolecularPlantSciences,ShanghaiInstituteofPlantPhysiologyandEcology,Chinese AcademyofSciences,Shanghai200032,China.
4.UniversityofChineseAcademyofSciences,Beijing100049,China.
5.DepartmentofMaterialsScienceand Engineering,SchoolofEngineering,SouthernUniversityofScienceandTechnology,Shenzhen518055,China.
6.Department of Biology, School of Life Sciences, Southern University of Science and Technology,Shenzhen 518055, China.
7.State Key Lab of Genetic Engineering & Institutes of Biomedical Sciences,Department of Microbiology and Microbial Engineering, School of Life Sciences,Fudan University,Shanghai200433,China.
8.Departmentof Chemistry,UniversityofPittsburgh,Pittsburgh,PA15260,USA.

Wei,Tan,Sihua,Cheng,Yingying,Li,et al. Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub[J]. NATURE COMMUNICATIONS,2022,13(1).

Wei,Tan.,Sihua,Cheng.,Yingying,Li.,Xiao-Yang,Li.,Ning,Lu.,...&Wei,Zhao.(2022).Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub.NATURE COMMUNICATIONS,13(1).

Wei,Tan,et al."Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub".NATURE COMMUNICATIONS 13.1(2022).