Title | Secukinumab attenuates neuroinflammation and neurobehavior defect via PKCβ/ERK/NF-κB pathway in a rat model of GMH |
Author | |
Corresponding Author | Gong,Ye; Tang,Jiping |
Publication Years | 2023-02-01
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DOI | |
Source Title | |
ISSN | 0014-4886
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EISSN | 1090-2430
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Volume | 360 |
Abstract | Aims: Germinal matrix hemorrhage (GMH) is a disastrous clinical event for newborns. Neuroinflammation plays an important role in the development of neurological deficits after GMH. The purpose of this study is to investigate the anti-inflammatory role of secukinumab after GMH and its underlying mechanisms involving PKCβ/ERK/NF-κB signaling pathway. Methods: A total of 154 Sprague-Dawley P7 rat pups were used. GMH was induced by intraparenchymal injection of bacterial collagenase. Secukinumab was administered intranasally post-GMH. PKCβ activator PMA and p-ERK activator Ceramide C6 were administered intracerebroventricularly at 24 h prior to GMH induction, respectively. Neurobehavioral tests, western blot and immunohistochemistry were used to evaluate the efficacy of Secukinumab in both short-term and long-term studies. Results: Endogenous IL-17A, IL-17RA, PKCβ and p-ERK were increased after GMH. Secukinumab treatment improved short- and long-term neurological outcomes, reduced the synthesis of MPO and Iba-1 in the perihematoma area, and inhibited the synthesis of proinflammatory factors, such as NF-κB, IL-1β, TNF-α and IL-6. Additionally, PMA and ceramide C6 abolished the beneficial effects of Secukinumab. Conclusion: Secukinumab treatment suppressed neuroinflammation and attenuated neurological deficits after GMH, which was mediated through the downregulation of the PKCβ/ERK/NF-κB pathway. Secukinumab treatment may provide a promising therapeutic strategy for GMH patients. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | First
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Funding Project | National Institutes of Health[NS082184];National Institutes of Health[NS101284];
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WOS Research Area | Neurosciences & Neurology
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WOS Subject | Neurosciences
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WOS Accession No | WOS:000895126300002
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Publisher | |
ESI Research Field | NEUROSCIENCE & BEHAVIOR
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Scopus EID | 2-s2.0-85142367083
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Data Source | Scopus
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Citation statistics |
Cited Times [WOS]:0
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/412530 |
Department | Shenzhen People's Hospital |
Affiliation | 1.Department of Pediatrics,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,China 2.Department of Critical Care Medicine,HuaShan Hospital,Fudan University,Shanghai,200040,China 3.Department of Physiology and Pharmacology,Center for Neuroscience Research,Loma Linda University School of Medicine,Loma Linda,92350,United States 4.Department of Neurosurgery,Loma Linda University School of Medicine,Loma Linda,92350,United States 5.Department of Anesthesiology,Loma Linda University School of Medicine,Loma Linda,92350,United States 6.Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai,200040,China 7.Department of Pediatrics,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Guangdong,China |
First Author Affilication | Shenzhen People's Hospital |
First Author's First Affilication | Shenzhen People's Hospital |
Recommended Citation GB/T 7714 |
Liu,Shengpeng,Deng,Shuixiang,Ding,Yan,et al. Secukinumab attenuates neuroinflammation and neurobehavior defect via PKCβ/ERK/NF-κB pathway in a rat model of GMH[J]. EXPERIMENTAL NEUROLOGY,2023,360.
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APA |
Liu,Shengpeng.,Deng,Shuixiang.,Ding,Yan.,Flores,Jerry J..,Zhang,Xiaoli.,...&Tang,Jiping.(2023).Secukinumab attenuates neuroinflammation and neurobehavior defect via PKCβ/ERK/NF-κB pathway in a rat model of GMH.EXPERIMENTAL NEUROLOGY,360.
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MLA |
Liu,Shengpeng,et al."Secukinumab attenuates neuroinflammation and neurobehavior defect via PKCβ/ERK/NF-κB pathway in a rat model of GMH".EXPERIMENTAL NEUROLOGY 360(2023).
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