Selective Formation of Osteogenic and Vasculogenic Tissues for Cartilage Regeneration

Liu，Zeyang1,2; Nan，Haochen1,2; Chiou，Yi Shiou3,4; Zhan，Zhen1,2; Lobie，Peter E.4; Hu，Chengzhi1,2

2022

10.1002/adhm.202202008

Advanced Healthcare Materials   Impact Factor & Quartile

2192-2640

2192-2659

Tissue-engineered periosteum substitutes (TEPSs) incorporating hierarchical architecture with osteoprogenitor and vascular niches are drawing much attention as a promising tool to support functional cells in defined zones and nourish the cortical bone. Current TEPSs usually lack technologies to closely observe cell performance, especially at the cell contact interface between distinct compartments containing defined biological configurations and functions. Here, an electrodeposition strategy is reported, which enables the selective formation of TEPSs with osteoprogenitor and vascular niches in a multiphasic scaffold in combination with different human cell types for cartilage regeneration in an in vivo osteochondral defect model. Human umbilical vein endothelial cells (HUVECs), dermal fibroblasts (HDFs), and bone marrow mesenchymal stem cells (hMSCs) are used to mirror both the vascular and osteogenic niches, respectively. It is observed that the intrinsic viscoelastic nature of the porous solid matrix is essential to successfully induce angiogenesis. Coculture of hMSCs with functional cells (HUVECs/HDFs) in TEPSs also effectively promoted periosteal regeneration, including osteogenic and angiogenic processes. The osteoarthritis cartilage histopathology assessment and histologic/histochemical grading system data indicate that the TEPSs containing hMSCs/HUVECs/HDFs exhibit superior potential for cartilage regeneration.

3D bone tissue cell patterning electrodeposition microenvironments regenerative periosteum

SCI

English

First ; Corresponding

National Natural Science Foundation of China[61903177] ; Shenzhen Science and Technology Program[JCYJ20190809144013494] ; Science and Technology Program of Guangdong[2021A1515011813] ; Science, Technology, and Innovation Commission of Shenzhen Municipality[ZDSYS20200811143601004] ; Shenzhen Development and Reform Commission Subject Construction Project[[2017]1434]

Engineering ; Science & Technology - Other Topics ; Materials Science

Engineering, Biomedical ; Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:000888882700001

WILEY

2-s2.0-85142346843

Scopus

1.Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems,Department of Mechanical and Energy Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Guangdong Provincial Key Laboratory of Human-Augmentation and Rehabilitation Robotics in Universities,Southern University of Science and Technology,Shenzhen,518055,China
3.Master Degree Program in Toxicology,College of Pharmacy,Kaohsiung Medical University,Kaohsiung,807,Taiwan
4.Tsinghua-Berkeley Shenzhen Institute (TBSI),Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,518055,China

Liu，Zeyang,Nan，Haochen,Chiou，Yi Shiou,et al. Selective Formation of Osteogenic and Vasculogenic Tissues for Cartilage Regeneration[J]. Advanced Healthcare Materials,2022.

Liu，Zeyang,Nan，Haochen,Chiou，Yi Shiou,Zhan，Zhen,Lobie，Peter E.,&Hu，Chengzhi.(2022).Selective Formation of Osteogenic and Vasculogenic Tissues for Cartilage Regeneration.Advanced Healthcare Materials.

Liu，Zeyang,et al."Selective Formation of Osteogenic and Vasculogenic Tissues for Cartilage Regeneration".Advanced Healthcare Materials (2022).