Dual GGDEF/EAL-Domain Protein RmcA Controls the Type III Secretion System of Pseudomonas aeruginosa by Interaction with CbrB

Kong，Weina1; Luo，Wei1; Wang，Yaya1; Liu，Yu1; Tian，Qianqian1; Zhao，Cheng1; Liang，Haihua1,2

2022

10.1021/acsinfecdis.2c00212

ACS Infectious Diseases   Impact Factor & Quartile

2373-8227

2373-8227

Cyclic diguanylate (c-di-GMP) is a major bacterial secondary signaling molecule that controls a multitude of cellular processes. More than 40 genes encoding diguanylate cyclases and phosphodiesterases have been identified in Pseudomonas aeruginosa, and many of them have been intensively investigated. However, the mechanism through which they achieve signaling specificity remains unclear. Here, we revealed that the absence of the dual GGDEF/EAL-domain protein RmcA significantly affected biofilm formation of P. aeruginosa PAO1 and led to upregulated expression of the type III secretion system (T3SS) genes; overexpression of RmcA strongly reduced the expression of T3SS. Further investigation showed that the regulatory function of RmcA was independent of the Gac/Rsm pathway. To identify the interaction partners of RmcA involved in this process, bacterial two-hybrid library screening was performed. We found that RmcA directly interacts with a two-component response regulator CbrB, which is involved in the regulation of biofilm formation and T3SS expression by RmcA. These findings reveal that the dual-domain GGDEF/EAL protein RmcA could achieve specificity of action through physical interaction with CbrB, which extends understanding the complex regulatory network of the c-di-GMP signaling.

biofilm formation c-di-GMP CbrB Pseudomonas aeruginosa RmcA type III secretion system

SCI

English

Corresponding

[32170188] ; [31870060] ; [31500111] ; [2019TD-016] ; [2020JM-424]

Pharmacology & Pharmacy ; Infectious Diseases

Chemistry, Medicinal ; Infectious Diseases

WOS:000886603600001

AMER CHEMICAL SOC

2-s2.0-85142208778

Scopus

1.Key Laboratory of Resources Biology and Biotechnology in Western China,Ministry of Education,School of Life Sciences,Northwest University,Xi'an,710069,China
2.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China

Kong，Weina,Luo，Wei,Wang，Yaya,et al. Dual GGDEF/EAL-Domain Protein RmcA Controls the Type III Secretion System of Pseudomonas aeruginosa by Interaction with CbrB[J]. ACS Infectious Diseases,2022.

Kong，Weina.,Luo，Wei.,Wang，Yaya.,Liu，Yu.,Tian，Qianqian.,...&Liang，Haihua.(2022).Dual GGDEF/EAL-Domain Protein RmcA Controls the Type III Secretion System of Pseudomonas aeruginosa by Interaction with CbrB.ACS Infectious Diseases.

Kong，Weina,et al."Dual GGDEF/EAL-Domain Protein RmcA Controls the Type III Secretion System of Pseudomonas aeruginosa by Interaction with CbrB".ACS Infectious Diseases (2022).