中文版 | English
Title

CRISPR/Cas systems: Delivery and application in gene therapy

Author
Corresponding AuthorJie Li; Aiping Lu; Chao Liang
Joint first authorJie Huang; Yitong Zhou
Publication Years
2022-11-22
DOI
Source Title
ISSN
2296-4185
Volume10
Abstract

The CRISPR/Cas systems in prokaryotes such as bacteria and archaea are the
adaptive immune system to prevent infection from viruses, phages, or other
foreign substances. When viruses or phages first invade the bacteria, Cas
proteins recognize and cut the DNA from viruses or phages into short
fragments that will be integrated into the CRISPR array. Once bacteria are
invaded again, the modified CRISPR and Cas proteins react quickly to cut DNA at
the specified target location, protecting the host. Due to its high efficiency,
versatility, and simplicity, the CRISPR/Cas system has become one of the most
popular gene editing technologies. In this review, we briefly introduce the
CRISPR/Cas systems, focus on several delivery methods including physical
delivery, viral vector delivery, and non-viral vector delivery, and the
applications of disease therapy. Finally, some problems in CRISPR/
Cas9 technology have been proposed, such as the off-target effects, the
efficiency of DNA repair mechanisms, and delivery of CRISPR/Cas system
safely and efficiently to the target location.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; 共同第一 ; Corresponding
Funding Project
Department of Education of Guangdong Province["82172386","81922081","82104216"] ; 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab)[2021KTSCX104] ; Guangdong Basic and Applied Basic Research Foundation[2020B1212030006] ; Science, Technology and Innovation Commission of Shenzhen["2020A1515011450","2022A1515012164"] ; [JCYJ20210324104201005] ; [JCYJ20190809094007719]
Funding Organization
National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL)
WOS Research Area
Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS Subject
Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS Accession No
WOS:000893948600001
Publisher
Data Source
人工提交
Publication Status
在线出版
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/415495
DepartmentDepartment of Biology
生命科学学院
Affiliation
1.Department of Biology, Southern University of Science and Technology, Shenzhen, China
2.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China
3.Institute of Integrated Bioinfomedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China
4.Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, China
5.Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou, China
6.State Key Laboratory of Proteomics, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China
First Author AffilicationDepartment of Biology;  School of Life Sciences
Corresponding Author AffilicationDepartment of Biology;  School of Life Sciences
First Author's First AffilicationDepartment of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Jie Huang,Yitong Zhou,Jie Li,et al. CRISPR/Cas systems: Delivery and application in gene therapy[J]. Frontiers in Bioengineering and Biotechnology,2022,10.
APA
Jie Huang,Yitong Zhou,Jie Li,Aiping Lu,&Chao Liang.(2022).CRISPR/Cas systems: Delivery and application in gene therapy.Frontiers in Bioengineering and Biotechnology,10.
MLA
Jie Huang,et al."CRISPR/Cas systems: Delivery and application in gene therapy".Frontiers in Bioengineering and Biotechnology 10(2022).
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