南方科技大学知识苑(SUSTech KC): CRISPR/Cas systems: Delivery and application in gene therapy

Title	CRISPR/Cas systems: Delivery and application in gene therapy
Author	Jie Huang1 ; Yitong Zhou 1; Jie Li 2; Aiping Lu 3,4,5; Chao Liang1,3,6
Corresponding Author	Jie Li; Aiping Lu; Chao Liang
Joint first author	Jie Huang; Yitong Zhou
Publication Years	2022-11-22
DOI	10.3389/fbioe.2022.942325
Source Title	Frontiers in Bioengineering and Biotechnology Impact Factor & Quartile
ISSN	2296-4185
Volume	10
Abstract	The CRISPR/Cas systems in prokaryotes such as bacteria and archaea are the adaptive immune system to prevent infection from viruses, phages, or other foreign substances. When viruses or phages first invade the bacteria, Cas proteins recognize and cut the DNA from viruses or phages into short fragments that will be integrated into the CRISPR array. Once bacteria are invaded again, the modified CRISPR and Cas proteins react quickly to cut DNA at the specified target location, protecting the host. Due to its high efficiency, versatility, and simplicity, the CRISPR/Cas system has become one of the most popular gene editing technologies. In this review, we briefly introduce the CRISPR/Cas systems, focus on several delivery methods including physical delivery, viral vector delivery, and non-viral vector delivery, and the applications of disease therapy. Finally, some problems in CRISPR/ Cas9 technology have been proposed, such as the off-target effects, the efficiency of DNA repair mechanisms, and delivery of CRISPR/Cas system safely and efficiently to the target location.
Keywords	CRISPR/Cas Systems, Delivery, Viral Vectors, Non-Viral Vectors, Genome Editing, Gene Therapy Delivery Viral Vectors Non-Viral Vectors Non-Viral Vectors Genome Editing Gene Therapy
URL	[Source Record]
Indexed By	SCI
Language	English
SUSTech Authorship	First ; 共同第一 ; Corresponding
Funding Project	Department of Education of Guangdong Province["82172386","81922081","82104216"] ; 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab)[2021KTSCX104] ; Guangdong Basic and Applied Basic Research Foundation[2020B1212030006] ; Science, Technology and Innovation Commission of Shenzhen["2020A1515011450","2022A1515012164"] ; [JCYJ20210324104201005] ; [JCYJ20190809094007719]
Funding Organization	National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; National Natural Science Foundation Council of China (82172386 and 81922081 to CL, 82104216 to JL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the Department of Education of Guangdong Province (2021KTSCX104 to CL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the 2020 Guangdong Provincial Science and Technology Innovation Strategy Special Fund (Guangdong-Hong Kong-Macau Joint Lab) (2020B1212030006 to AL) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Guangdong Basic and Applied Basic Research Foundation (2020A1515011450 to JL and 2022A1515012164) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL) ; the Science, Technology and Innovation Commission of Shenzhen (JCYJ20210324104201005 to CL and JCYJ20190809094007719 to JL)
WOS Research Area	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS Subject	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS Accession No	WOS:000893948600001
Publisher	FRONTIERS MEDIA SA
Data Source	人工提交
Publication Status	在线出版
Citation statistics	Cited Times [WOS]:2
Document Type	Journal Article
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/415495
Department	Department of Biology 生命科学学院
Affiliation	1.Department of Biology, Southern University of Science and Technology, Shenzhen, China 2.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China 3.Institute of Integrated Bioinfomedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China 4.Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, China 5.Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou, China 6.State Key Laboratory of Proteomics, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China
First Author Affilication	Department of Biology; School of Life Sciences
Corresponding Author Affilication	Department of Biology; School of Life Sciences
First Author's First Affilication	Department of Biology; School of Life Sciences
Recommended Citation GB/T 7714	Jie Huang,Yitong Zhou,Jie Li,et al. CRISPR/Cas systems: Delivery and application in gene therapy[J]. Frontiers in Bioengineering and Biotechnology,2022,10.
APA	Jie Huang,Yitong Zhou,Jie Li,Aiping Lu,&Chao Liang.(2022).CRISPR/Cas systems: Delivery and application in gene therapy.Frontiers in Bioengineering and Biotechnology,10.
MLA	Jie Huang,et al."CRISPR/Cas systems: Delivery and application in gene therapy".Frontiers in Bioengineering and Biotechnology 10(2022).

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