Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions
|Corresponding Author||Bo,Li; Chuang,Xue|
|Joint first author||Wei,Kang; Xiao,Ma|
Cells use self-assembled biomaterials such as lipid membranes or proteinaceous shells to coordinate thousands of reactions that simultaneously take place within crowded spaces. However, mimicking such spatial organization for synthetic applications in engineered systems remains a challenge, resulting in inferior catalytic efficiency. In this work, we show that protein cages as an ideal scaffold to organize enzymes to enhance cascade reactions both in vitro and in living cells. We demonstrate that not only enzyme-enzyme distance but also the improved Km value contribute to the enhanced reaction rate of cascade reactions. Three sequential enzymes for lycopene biosynthesis have been co-localized on the exterior of the engineered protein cages in Escherichia coli, leading to an 8.5-fold increase of lycopene production by streamlining metabolic flux towards its biosynthesis. This versatile system offers a powerful tool to achieve enzyme spatial organization for broad applications in biocatalysis.
NI Journal Papers
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Cited Times [WOS]:1
|Document Type||Journal Article|
|Department||Department of Biomedical Engineering|
1.State Key Laboratory of Fine Chemicals, Frontiers Science Centre for Smart Materials Oriented Chemical Engineering, School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
2.Ningbo Institute of Dalian University of Technology, Ningbo, 315016, China.
3.Department of Mechanical Engineering, Kennesaw State University, GA 0060, Marietta, US.
4.Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
5.National Energy Research Centre for Biorefinery, Beijing University of Chemical Technology, Beijing, 100029, China.
6.School of Biomedical Engineering, SUN YAT-SEN University, Shenzhen, 518000, China.
Wei,Kang,Xiao,Ma,Deepika,Kakarla,等. Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022.
Wei,Kang.,Xiao,Ma.,Deepika,Kakarla.,Huawei,Zhang.,Yunming,Fang.,...&Chuang,Xue.(2022).Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
Wei,Kang,et al."Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022).
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