中文版 | English
Title

Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions

Author
Corresponding AuthorBo,Li; Chuang,Xue
Joint first authorWei,Kang; Xiao,Ma
Publication Years
2022-10-26
DOI
Source Title
ISSN
1433-7851
EISSN
1521-3773
Abstract

Cells use self-assembled biomaterials such as lipid membranes or proteinaceous shells to coordinate thousands of reactions that simultaneously take place within crowded spaces. However, mimicking such spatial organization for synthetic applications in engineered systems remains a challenge, resulting in inferior catalytic efficiency. In this work, we show that protein cages as an ideal scaffold to organize enzymes to enhance cascade reactions both in vitro and in living cells. We demonstrate that not only enzyme-enzyme distance but also the improved Km value contribute to the enhanced reaction rate of cascade reactions. Three sequential enzymes for lycopene biosynthesis have been co-localized on the exterior of the engineered protein cages in Escherichia coli, leading to an 8.5-fold increase of lycopene production by streamlining metabolic flux towards its biosynthesis. This versatile system offers a powerful tool to achieve enzyme spatial organization for broad applications in biocatalysis.

Keywords
URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
Others
WOS Research Area
Chemistry
WOS Subject
Chemistry, Multidisciplinary
WOS Accession No
WOS:000888994600001
Publisher
ESI Research Field
CHEMISTRY
Data Source
人工提交
Publication Status
在线出版
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/415990
DepartmentDepartment of Biomedical Engineering
Affiliation
1.State Key Laboratory of Fine Chemicals, Frontiers Science Centre for Smart Materials Oriented Chemical Engineering, School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
2.Ningbo Institute of Dalian University of Technology, Ningbo, 315016, China.
3.Department of Mechanical Engineering, Kennesaw State University, GA 0060, Marietta, US.
4.Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
5.National Energy Research Centre for Biorefinery, Beijing University of Chemical Technology, Beijing, 100029, China.
6.School of Biomedical Engineering, SUN YAT-SEN University, Shenzhen, 518000, China.
Recommended Citation
GB/T 7714
Wei,Kang,Xiao,Ma,Deepika,Kakarla,等. Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022.
APA
Wei,Kang.,Xiao,Ma.,Deepika,Kakarla.,Huawei,Zhang.,Yunming,Fang.,...&Chuang,Xue.(2022).Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
MLA
Wei,Kang,et al."Organizing Enzymes on Self‐Assembled Protein Cages for Cascade Reactions".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022).
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