中文版 | English
Title

Comprehensive mapping of alternative polyadenylation site usage and its dynamics at single-cell resolution

Author
Corresponding AuthorQi Yuanming; Hong Ni; Ni Ting; Jin Wenfei
Joint first authorWang Junliang; Chen Wei
Publication Years
2022-12-01
DOI
Source Title
ISSN
0027-8424
EISSN
1091-6490
Volume119Issue:49
Abstract
Alternative polyadenylation (APA) plays an important role in posttranscriptional gene regulation such as transcript stability and translation efficiency. However, our knowledge about APA dynamics at the single-cell level is largely unexplored. Here, we developed single-cell polyadenylation sequencing, a strand-specific approach for sequencing the 3' end of transcripts, to investigate the landscape of APA at the single-cell level. By analyzing several cell lines, we found many genes using multiple polyA sites in bulk data are prone to use only one polyA site in each single cell. Interestingly, cell cycle genes were significantly enriched in genes with high variation in polyA site usages. Furthermore, the 414 genes showing a polyA site usage switch after cell synchronization enriched cell cycle genes, while the differentially expressed genes after cell synchronization did not enrich cell cycle genes. We further identified 812 genes showing polyA site usage changes between neighboring cell cycles, which were grouped into six clusters, with cell phase-specific functional categories enriched in each cluster. Deletion of one polyA site in MSL1 and SCCPDH results in slower and faster cell cycle progression, respectively, supporting polyA site usage switch played an important role in cell cycle. These results indicate that APA is an important layer for cell cycle regulation.
Keywords
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Indexed By
Language
English
Important Publications
NI Journal Papers ; NI论文
SUSTech Authorship
Corresponding
Funding Project
National Key Research and Development Program of China["2021YFA0909300","2021YFF1200900"] ; National Natural Science Foundation of China[32170646] ; Shenzhen Science and Technology Program[KQTD20180411143432337] ; Shenzhen Innovation Committee of Science and Technology["JCYJ20180504170158430","JCYJ20220818100401003","ZDSYS20200811144002008"]
WOS Research Area
Science & Technology - Other Topics
WOS Subject
Multidisciplinary Sciences
WOS Accession No
WOS:000972701200001
Publisher
ESI Research Field
BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/416231
DepartmentSchool of Life Sciences
生命科学学院_生物系
Affiliation
1.School of Life Sciences, Zhengzhou University
2.Shenzhen Key Laboratory of Gene Regulation and Systems Biology, School of Life Sciences, Southern University of Science and Technology,
3.State Key Laboratory of Genetic Engineering & Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Shanghai Cancer Center, Fudan University,
First Author AffilicationSchool of Life Sciences
Corresponding Author AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Wang Junliang,Chen Wei,Yue Wenjun,et al. Comprehensive mapping of alternative polyadenylation site usage and its dynamics at single-cell resolution[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(49).
APA
Wang Junliang.,Chen Wei.,Yue Wenjun.,Hou Wenhong.,Rao Feng.,...&Jin Wenfei.(2022).Comprehensive mapping of alternative polyadenylation site usage and its dynamics at single-cell resolution.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(49).
MLA
Wang Junliang,et al."Comprehensive mapping of alternative polyadenylation site usage and its dynamics at single-cell resolution".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.49(2022).
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