中文版 | English
Title

The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus

Author
Corresponding AuthorZhang, Weimin; Zhang, Lihong
Publication Years
2022-11-23
DOI
Source Title
EISSN
2056-3132
Volume7Issue:1
Abstract
Background The ricefield eel is a freshwater protogynous hermaphrodite fish and has become an important aquaculture species in China. The sex change of ricefield eel is impeding its aquaculture practice, particularly the large-scale artificial breeding. Many studies including transcriptomes of mixed gonadal samples from different individuals have been aimed to elucidate mechanisms underlying the sex change. However, the key physiological factors involved in the initiation of sex change remain to be identified. Results The present study performed transcriptomic analysis on gonadal samples of different sexual stages obtained through biopsy from the same fish undergoing sex change. A total of 539,764,816 high-quality reads were generated from twelve cDNA libraries of gonadal tissues at female (F), early intersexual (EI), mid-intersexual (MI), and late intersexual (LI) stages of three individual sex-changing fish. Pairwise comparisons between EI and F, MI and EI, and LI and MI identified 886, 319, and 10,767 differentially expressed genes (DEGs), respectively. Realtime quantitative PCR analysis of 12 representative DEGs showed similar expression profiles to those inferred from transcriptome data, suggesting the reliability of RNA-seq data for gene expression analysis. The expression of apoeb, csl2, and enpp2 was dramatically increased and peaked at EI while that of cyp19a1a, wnt4a, fgf16, and foxl2a significantly downregulated from F to EI and remained at very low levels during subsequent development until LI, which suggests that apoeb, csl2, enpp2, cyp19a1a, wnt4a, fgf16, and foxl2a may be closely associated with the initiation of sex change of ricefield eels. Conclusions Collectively, results of the present study confirmed that the down-regulation of female-related genes, such as cyp19a1a, wnt4a, fgf16, and foxl2a, is important for the sex change of ricefield eels. More importantly, some novel genes, including apoeb, csl2, and enpp2, were shown to be expressed with peak values at EI, which are potentially involved in the initiation of sex change. The present transcriptomic data may provide an important research resource for further unraveling the mechanisms underlying the sex change and testicular development in ricefield eels as well as other teleosts.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
National Key Research and Development Program[2018YFD0900203] ; National Natural Science Foundation of China["32072956","31572604"] ; Project of Guangdong Basic and Applied Basic Research Foundation[2020A1515111185]
WOS Research Area
Zoology
WOS Subject
Zoology
WOS Accession No
WOS:000886971100001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/417041
DepartmentShenzhen People's Hospital
Affiliation
1.Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, Guangzhou, Peoples R China
2.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Inst Biomed Engn, Shenzhen 518020, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
5.Sun Yat Sen Univ, Sch Life Sci, Biol Dept, Guangzhou, Peoples R China
Recommended Citation
GB/T 7714
Fan, Miao,Yang, Wei,Zhang, Weimin,et al. The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus[J]. BMC ZOOLOGY,2022,7(1).
APA
Fan, Miao,Yang, Wei,Zhang, Weimin,&Zhang, Lihong.(2022).The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus.BMC ZOOLOGY,7(1).
MLA
Fan, Miao,et al."The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus".BMC ZOOLOGY 7.1(2022).
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