Title | Downregulation of miR-26b attenuates early brain injury induced by subarachnoid hemorrhage via mediating the KLF4/STAT3/HMGB1 axis |
Author | |
Corresponding Author | Dai, Limeng; Wang, Hao |
Publication Years | 2023
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DOI | |
Source Title | |
ISSN | 0014-4886
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EISSN | 1090-2430
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Volume | 359 |
Abstract | Background: Early brain injury (EBI) refers to early-onset secondary complications that occur after subarachnoid hemorrhage (SAH), and associated with high rate of disability and mortality. Recent investigations have indi-cated microRNA-26b (miR-26b) as a biomarker in the progression of SAH. Accordingly, the present study was designed to elucidate the role of miR-26b in influencing EBI following SAH and the downstream mechanisms.Methods: Firstly, SAH rat models and neuron injury models were developed to assess the effect of miR-26b on EBI-like symptoms and subsequent inflammation. Dual-luciferase reporter gene assay was further implemented to evaluate the binding of miR-26b to its putative target gene STAT3. Loss-of-function and rescue experiments were performed to assess the functionality of miR-26b-mediated STAT3 in both models.Results: miR-26b was found to target KLF4 and negative-modulate its expression, whereby aggravating EBI and inflammatory response in SAH rat models and stimulating hemoglobin-induced apoptosis in astrocytes. On the other hand, silencing of miR-26b reversed these changes in SAH rat models and hemoglobin (Hb)-induced as-trocytes. miR-26b could further activate STAT3 via down-regulation of KLF4. Furthermore, KLF4 knockdown up -regulated HMGB1 to aggravate EBI following SAH.Conclusions: Collectively, our findings highlighted the ameliorative effect of miR-26b inhibition on EBI in SAH and the possible mechanism associated with the KLF4/STAT3/HMGB1 axis. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | First
; Corresponding
|
Funding Project | [ZDSYS20200811142804014]
; [SZXK045]
; [JCYJ20210324114008023]
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WOS Research Area | Neurosciences & Neurology
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WOS Subject | Neurosciences
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WOS Accession No | WOS:000890330600003
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Publisher | |
ESI Research Field | NEUROSCIENCE & BEHAVIOR
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Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:1
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/417058 |
Department | Shenzhen People's Hospital |
Affiliation | 1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Crit Care Med,Clin Med Coll 2,Affiliated Hosp, Shenzhen 518020, Peoples R China 2.Shijiazhuang Huayao Hosp North China Med & Hlth Gr, Dept Neurol, Shijiazhuang 050000, Peoples R China 3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Neurosurg,Clin Med Coll 2,Jinan Univ, Shenzhen 518020, Peoples R China 4.Guangdong Engn Technol Res Ctr Nervous Anat & Rela, Shenzhen 518020, Peoples R China 5.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Neurosurg, Clin Med Coll 2,Jinan Univ,Affiliated Hosp 1, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China |
First Author Affilication | Shenzhen People's Hospital |
Corresponding Author Affilication | Shenzhen People's Hospital |
First Author's First Affilication | Shenzhen People's Hospital |
Recommended Citation GB/T 7714 |
Huang, Zewei,Liu, Jialin,Xu, Jiongfu,et al. Downregulation of miR-26b attenuates early brain injury induced by subarachnoid hemorrhage via mediating the KLF4/STAT3/HMGB1 axis[J]. EXPERIMENTAL NEUROLOGY,2023,359.
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APA |
Huang, Zewei,Liu, Jialin,Xu, Jiongfu,Dai, Limeng,&Wang, Hao.(2023).Downregulation of miR-26b attenuates early brain injury induced by subarachnoid hemorrhage via mediating the KLF4/STAT3/HMGB1 axis.EXPERIMENTAL NEUROLOGY,359.
|
MLA |
Huang, Zewei,et al."Downregulation of miR-26b attenuates early brain injury induced by subarachnoid hemorrhage via mediating the KLF4/STAT3/HMGB1 axis".EXPERIMENTAL NEUROLOGY 359(2023).
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