Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer

Wang, Daxuan1; Feng, Ziyang2; Lin, Changjian1; Xie, Tianjian3; Li, Wujin4; Wu, Famiao3; Chen, Zhengwei1; Wang, Yan5; Huang, Jianxin6

2022-08-01

10.23812/j.biol.regul.homeost.agents.20223604.121

JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS   Impact Factor & Quartile

0393-974X

1724-6083

Background: Tumor-specific neoantigens are ideal targets for cancer immunotherapy, but few of these have been identified thus far. An analysis strategy was developed for neoantigen in non-coding regions by RNC (ribosome-nascent chain complex)-RNAseq in mouse lung cancer. Methods: In LLC (Lewis lung carcinoma) cell lines, RNC extraction, RNC-RNA extraction, RNC-RNA seq and bioinformatics analysis were carried out. The immunogenicity and specificity were determined by immunizing mice. Results: Twenty neoantigens were identified from non-coding regions. Seventeen long peptides were synthesized successfully and 5/17 of these neoantigens were found to be immunogenic. In tumor transplant models, the non-coding neoantigen vaccines immunization achieved in vivo tumor control in protective and therapeutic settings. Conclusions: In mice, the non-coding neoantigen vaccines can provoke anti-tumour effect against lung cancer. This can form the basis for future study of RNC-RNA-seq, as a method to identify non-coding neoantigens.

non-coding neoantigens RNC (ribosome-nascent chain complex)-RNA lung cancer immunotherapy mouse

SCI

English

Others

Fujian Medical University, China[2017XQ1129] ; Fujian Provincial Health Science and Technology Project, China[2020GGB002] ; Guangdong Medical Science and Technology Research Foundation[A2020487]

Endocrinology & Metabolism ; Immunology ; Research & Experimental Medicine ; Physiology

Endocrinology & Metabolism ; Immunology ; Medicine, Research & Experimental ; Physiology

WOS:000891806500001

BIOLIFE SAS

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Resp & Crit Care Med, Fuzhou 350000, Fujian, Peoples R China
2.Second Mil Med Univ, Shanghai Changhai Hosp, Affiliated Hosp 1, Dept Transfus Med, Shanghai 200000, Peoples R China
3.Xiapu Hosp, Dept Resp, Ningde 352000, Fujian, Peoples R China
4.Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Thorac Surg, Fuzhou 350000, Fujian, Peoples R China
5.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Sch 2,Dept Resp & Crit Care, Shenzhen 518000, Guangdong, Peoples R China
6.Fujian Med Univ, Fujian Prov Hosp, Shengli Clin Med Coll, Dept Clin Lab, Fuzhou, Chin, Myanmar

Wang, Daxuan,Feng, Ziyang,Lin, Changjian,et al. Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer[J]. JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,2022,36(4).

Wang, Daxuan.,Feng, Ziyang.,Lin, Changjian.,Xie, Tianjian.,Li, Wujin.,...&Huang, Jianxin.(2022).Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer.JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,36(4).

Wang, Daxuan,et al."Identification of Neoantigen from Non-Coding Region by RNC-RNA-Sequence in Mouse Lung Cancer".JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 36.4(2022).