A PEG-lipid-free COVID-19 mRNA vaccine triggers robust immune responses in mice

Li, Min1,2; Huang, Yixuan1,2; Wu, Jiacai1,2,3; Li, Sanpeng1,2; Mei, Miao4; Chen, Haixia2,5; Wang, Ning1,2,3; Wu, Weigang1,2; Zhou, Boping1,2,3; Tan, Xu2,4; Li, Bin1,2,3

2022-11-01

10.1039/d2mh01260j

Materials Horizons   Impact Factor & Quartile

2051-6347

2051-6355

COVID-19 mRNA vaccines represent a completely new category of vaccines and play a crucial role in controlling the COVID-19 pandemic. In this study, we have developed a PEG-lipid-free two-component mRNA vaccine (PFTCmvac) by formulating mRNA encoding the receptor binding domain (RBD) of SARS-CoV-2 into lipid-like nanoassemblies. Without using polyethylene glycol (PEG)-lipids, the self-assembled PFTCmvac forms thermostable nanoassemblies and exhibits a dose-dependent cellular uptake and membrane disruption, eventually leading to high-level protein expression in both mammalian cells and mice. Vaccination with PFTCmvac elicits strong humoral and cellular responses in mice, without evidence of significant adverse reactions. In addition, the vaccine platform does not trigger complement activation in human serum, even at a high serum concentration. Collectively, the PEG-lipid-free two-component nanoassemblies provide an alternative delivery technology for COVID-19 mRNA vaccines and opportunities for the rapid production of new mRNA vaccines against emerging infectious diseases.

SCI

English

First ; Corresponding

[82072053] ; [32100107] ; [RCYX20200714114539061] ; [JCYJ20190806150018265] ; [20211080036]

Chemistry ; Materials Science

Chemistry, Multidisciplinary ; Materials Science, Multidisciplinary

WOS:000893827500001

ROYAL SOC CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Infect Dis, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
2.Jinan Univ, Clin Med Coll 2, Shenzhen 518020, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
4.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Pharmaceut Sci, Beijing 100084, Peoples R China
5.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Clin Lab, Affiliated Hosp 1, Shenzhen 518020, Peoples R China

Li, Min,Huang, Yixuan,Wu, Jiacai,et al. A PEG-lipid-free COVID-19 mRNA vaccine triggers robust immune responses in mice[J]. Materials Horizons,2022.

Li, Min.,Huang, Yixuan.,Wu, Jiacai.,Li, Sanpeng.,Mei, Miao.,...&Li, Bin.(2022).A PEG-lipid-free COVID-19 mRNA vaccine triggers robust immune responses in mice.Materials Horizons.

Li, Min,et al."A PEG-lipid-free COVID-19 mRNA vaccine triggers robust immune responses in mice".Materials Horizons (2022).