中文版 | English
Title

Reversal of Peripheral Neuropathic Pain by the Small-Molecule Natural Product Narirutin via Block of Na(v)1.7 Voltage-Gated Sodium Channel

Author
Corresponding AuthorCheung, Chi Wai; Cai, Song
Publication Years
2022-12-01
DOI
Source Title
EISSN
1422-0067
Volume23Issue:23
Abstract
Neuropathic pain is a refractory chronic disease affecting millions of people worldwide. Given that present painkillers have poor efficacy or severe side effects, developing novel analgesics is badly needed. The multiplex structure of active ingredients isolated from natural products provides a new source for phytochemical compound synthesis. Here, we identified a natural product, Narirutin, a flavonoid compound isolated from the Citrus unshiu, showing antinociceptive effects in rodent models of neuropathic pain. Using calcium imaging, whole-cell electrophysiology, western blotting, and immunofluorescence, we uncovered a molecular target for Narirutin's antinociceptive actions. We found that Narirutin (i) inhibits Veratridine-triggered nociceptor activities in L4-L6 rat dorsal root ganglion (DRG) neurons, (ii) blocks voltage-gated sodium (Na-V) channels subtype 1.7 in both small-diameter DRG nociceptive neurons and human embryonic kidney (HEK) 293 cell line, (iii) does not affect tetrodotoxin-resistant (TTX-R) Na-V channels, and (iv) blunts the upregulation of Na(v)1.7 in calcitonin gene-related peptide (CGRP)-labeled DRG sensory neurons after spared nerve injury (SNI) surgery. Identifying Na(v)1.7 as a molecular target of Narirutin may further clarify the analgesic mechanism of natural flavonoid compounds and provide an optimal idea to produce novel selective and efficient analgesic drugs.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
WOS Research Area
Biochemistry & Molecular Biology ; Chemistry
WOS Subject
Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS Accession No
WOS:000896129100001
Publisher
ESI Research Field
CHEMISTRY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/417095
DepartmentShenzhen People's Hospital
Affiliation
1.Shenzhen Univ, Dept Anat & Histol, Hlth Sci Ctr, Shenzhen 518060, Peoples R China
2.Univ Hong Kong, Lab & Clin Res Inst Pain, Li Ka Shing Fac Med, Sch Clin Med,Dept Anaesthesiol, Hong Kong 999077, Peoples R China
3.Southern Univ Sci & Technol, Dept Anethesiol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
4.Jinan Univ, Clin Med Coll 2, Shenzhen 518020, Peoples R China
5.Shenzhen Engn Res Ctr Anesthesiol, Shenzhen 518020, Peoples R China
6.City Univ Hong Kong, Dept Neurosci, Hong Kong 999077, Peoples R China
Recommended Citation
GB/T 7714
Yang, Haoyi,Shan, Zhiming,Guo, Weijie,et al. Reversal of Peripheral Neuropathic Pain by the Small-Molecule Natural Product Narirutin via Block of Na(v)1.7 Voltage-Gated Sodium Channel[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(23).
APA
Yang, Haoyi.,Shan, Zhiming.,Guo, Weijie.,Wang, Yuwei.,Cai, Shuxian.,...&Cai, Song.(2022).Reversal of Peripheral Neuropathic Pain by the Small-Molecule Natural Product Narirutin via Block of Na(v)1.7 Voltage-Gated Sodium Channel.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(23).
MLA
Yang, Haoyi,et al."Reversal of Peripheral Neuropathic Pain by the Small-Molecule Natural Product Narirutin via Block of Na(v)1.7 Voltage-Gated Sodium Channel".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.23(2022).
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