| Title | Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis |
| Author | |
| Corresponding Author | Liu, Dongzhou; Hong, Xiaoping |
| Publication Years | 2022-12-01
|
| DOI | |
| Source Title | |
| ISSN | 1535-3893
|
| EISSN | 1535-3907
|
| Abstract | Dermatomyositis and polymyositis (DM/PM) are systemic autoimmune diseases characterized by proximal muscle weakness. The underlying pathogenetic mechanism of this disease remains under-researched. Here, using proteomics analysis, a great overlap of differentially expressed plasma exosomal proteins involved in the complement and coagulation cascade pathway, including FGA, FGB, FGG, C1QB, C1QC, and VWF, was identified in DM/PM patients versus healthy controls. Correlation analysis showed that the expression levels of complement-associated proteins (C1QB and C1QC) correlated positively with CRP, ESR, and platelet count. ROC curve analysis demonstrated that complement and coagulation cascade-associated proteins could be strong predictors for DM/ PM. In addition, we also identified several other proteins that were differentially expressed in DM and PM. The selected candidate proteins were further validated by parallel reaction monitoring (PRM) and enzyme-linked immunosorbent assay (ELISA). Together, our findings indicate that these exosome-derived proteins might participate in microvascular damage in DM/PM through the activation of the complement and coagulation cascade pathway and function as biomarkers for the clinical diagnosis of DM/PM. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | [SZXK011]
; [SZSM202111006]
|
| WOS Research Area | Biochemistry & Molecular Biology
|
| WOS Subject | Biochemical Research Methods
|
| WOS Accession No | WOS:000895914800001
|
| Publisher | |
| ESI Research Field | BIOLOGY & BIOCHEMISTRY
|
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:0
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/417098 |
| Department | Shenzhen People's Hospital |
| Affiliation | Southern Univ Sci & Technol, Jinan Univ, Dept Rheumatol & Immunol, Shenzhen Peoples Hosp,Clin Med Coll 2,Affiliated H, Shenzhen 518020, Guangdong, Peoples R China |
| First Author Affilication | Shenzhen People's Hospital |
| Corresponding Author Affilication | Shenzhen People's Hospital |
| First Author's First Affilication | Shenzhen People's Hospital |
| Recommended Citation GB/T 7714 |
Meng, Shuhui,Wang, Tingting,Zhao, Qianqian,et al. Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis[J]. JOURNAL OF PROTEOME RESEARCH,2022.
|
| APA |
Meng, Shuhui.,Wang, Tingting.,Zhao, Qianqian.,Hu, Qiu.,Chen, Yulan.,...&Hong, Xiaoping.(2022).Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis.JOURNAL OF PROTEOME RESEARCH.
|
| MLA |
Meng, Shuhui,et al."Proteomics Analysis of Plasma-Derived Exosomes Unveils the Aberrant Complement and Coagulation Cascades in Dermatomyositis/Polymyositis".JOURNAL OF PROTEOME RESEARCH (2022).
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