Rapid, sensitive, and high-throughput quantification of broad serological ceramides by using isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry

Chen, Shuailong1,2; Li, Xuan2; Liu, Shijia3; Zhao, Longshan1; Zhang, Wenyong2; Xiong, Zhili1; Luan, Hemi2,4

2022-12-01

10.1007/s00216-022-04473-x

ANALYTICAL AND BIOANALYTICAL CHEMISTRY   Impact Factor & Quartile

1618-2642

1618-2650

Ceramides are important intermediates in the metabolism of sphingolipids. High-throughput liquid chromatography-mass spectrometry has been used extensively for monitoring the levels of serological ceramides, but is still limited by inadequate coverage or lack of sensitivity. Herein, a rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed and verified for accurate quantification of 41 ceramides, involving ceramides with C16-20 sphingosine, dihydro-ceramide, and dehydro-ceramide. This method was validated with excellent linearity (R-2 > 0.99) and good recovery in the range of 90-110%. Intra- and inter-day imprecision were below 5.57% and 7.83% respectively. The improved high-throughput quantitative method developed in this study may aid in the accurate characterization of ceramides for understanding ceramide biology and application in disease diagnosis.

High-throughput Liquid chromatography Tandem mass spectrometry Ceramide

SCI

English

Corresponding

[21904058]

Biochemistry & Molecular Biology ; Chemistry

Biochemical Research Methods ; Chemistry, Analytical

WOS:000895273300001

SPRINGER HEIDELBERG

CHEMISTRY

Web of Science

1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Liaoning, Peoples R China
2.Southern Univ Sci & Technol, Acad Adv Interdisciplinary Studies, Sch Med, 1088 Xueyuan Rd, Shenzhen, Peoples R China
3.Nanjing Univ Chinese Med, Affiliated Hosp, Nanjing, Jiangsu, Peoples R China
4.Tsinghua Shenzhen Int Grad Sch, State Key Lab Chem Oncogen, Shenzhen, Peoples R China

Chen, Shuailong,Li, Xuan,Liu, Shijia,et al. Rapid, sensitive, and high-throughput quantification of broad serological ceramides by using isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022.

Chen, Shuailong.,Li, Xuan.,Liu, Shijia.,Zhao, Longshan.,Zhang, Wenyong.,...&Luan, Hemi.(2022).Rapid, sensitive, and high-throughput quantification of broad serological ceramides by using isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.

Chen, Shuailong,et al."Rapid, sensitive, and high-throughput quantification of broad serological ceramides by using isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022).