Minimally invasive microglial and neuronal imaging in mouse spinal cord dorsal horn

Wanjie Wu1; Sicong He2; Yujun Chen3; Congping Chen1; Yiming Fu1; Kai Liu4; Jianan Y. Qu4

2022

10.1109/JSTQE.2022.3229705

IEEE Journal of Selected Topics in Quantum Electronics   Impact Factor & Quartile

1558-4542

1558-4542

The spinal cord dorsal horn is a relay hub that receives sensory information from the peripheral nervous system and transmits bioelectrical signals to the brain. In vivo imaging of neuronal and glial activity in the dorsal horn can provide insights into both functions and dysfunctions of the neuronal network in the spinal cord. With multimodal NLO microscopy, we identified a thin-myelin sheath region allowing to image over 200 μm deep below pia with subcellular resolution. By using an optically cleared intervertebral window, dorsal horn neuron and microglia activities can be observed without activating spinal cord inflammation. Two-photon imaging of neurons and microglia as well as the optical clearing improvement at different tissue depths were further characterized over time. Using this inflammation-free imaging method, we conducted a longitudinal study of dorsal horn microglia dynamics following sciatic nerve transection. Furthermore, stable in vivo calcium imaging of neurons in the dorsal horn was performed with electrical stimulation on the mouse's hind paw. The subcellular-resolution imaging enabled characterization of the distinct calcium transients of neuronal somas and dendrites. This minimally invasive imaging approach to spinal dorsal horn through an optically cleared intervertebral window provides a reliable platform for studying and understanding cellular activities in the spinal dorsal horn.

In Vivo imagIng Spinal Cord Nonlinear Optical Microscopy Optical Clearing Microglia Calcium Imaging

SCI

English

Others

National Science Foundation of China["32192400","32101211"] ; Hong Kong Research Grants Council["16102518","16102920","16102421","16102122","T13-607/12R","T13-706/11-1","T13-605/18W","C6001-19E","N_HKUST603/19"] ; Innovation and Technology Commission[ITCPD/17-9] ; Area of Excellence Scheme of the University["AoE/M-604/16","AOE/M-09/12"]

Engineering ; Physics ; Optics

Engineering, Electrical & Electronic ; Quantum Science & Technology ; Optics ; Physics, Applied

WOS:000926634500001

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

2-s2.0-85144779868

IEEE

1.Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. China
2.Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China
3.Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. China
4.Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P.R. China

Wanjie Wu,Sicong He,Yujun Chen,et al. Minimally invasive microglial and neuronal imaging in mouse spinal cord dorsal horn[J]. IEEE Journal of Selected Topics in Quantum Electronics,2022,PP(99):1-17.

Wanjie Wu.,Sicong He.,Yujun Chen.,Congping Chen.,Yiming Fu.,...&Jianan Y. Qu.(2022).Minimally invasive microglial and neuronal imaging in mouse spinal cord dorsal horn.IEEE Journal of Selected Topics in Quantum Electronics,PP(99),1-17.

Wanjie Wu,et al."Minimally invasive microglial and neuronal imaging in mouse spinal cord dorsal horn".IEEE Journal of Selected Topics in Quantum Electronics PP.99(2022):1-17.