Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae

Zhang, Jianzhi1,3; Li, Tuo1,3; Hong, Zhilai1,3; Ma, Chenfei1; Fang, Xiaoting1; Zheng, Fengfeng2,3; Teng, Wenkai2,3; Zhang, Chuanlun2,3; Si, Tong1,3

2022-12-01

10.1002/anie.202214344

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Impact Factor & Quartile

1433-7851

1521-3773

Discovery of the Asgard superphylum of archaea provides new evidence supporting the two-domain model of life: eukaryotes originated from an Asgard-related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial-like membrane lipids with a sn-glycerol-3-phosphate (G3P) backbone instead of the archaeal-like sn-glycerol-1-phosphate (G1P) backbone remains unknown. In this study, we reconstituted archaeal lipid production in Saccharomyces cerevisiae by expressing unsaturated archaeol-synthesizing enzymes. Using Golden Gate cloning for pathway assembly, modular gene replacement was performed, revealing the potential biosynthesis of both G1P- and G3P-based unsaturated archaeol by uncultured Asgard archaea. Unexpectedly, hybrid neutral lipids containing both archaeal isoprenoids and eukaryotic fatty acids were observed in recombinant S. cerevisiae. The ability of yeast and archaeal diacylglycerol acyltransferases to synthesize such hybrid lipids was demonstrated.

Chirality Diacylglycerol Acyltransferases Hybrid Neutral Lipids Synthetic Biology

SCI

English

NI Journal Papers

Others

National Key Research and Development Program of China["2021YFA0910800","2020YFA0908500"] ; National Natural Science Foundation of China["32101179","32071428","91851210"] ; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[K19313901] ; Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Southern University of Science and Technology[ZDSYS201802081843490]

Chemistry

Chemistry, Multidisciplinary

WOS:000898260100001

WILEY-V C H VERLAG GMBH

CHEMISTRY

Web of Science

1.Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, 1068 XueYuan Ave, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Dept Ocean Sci & Engn, Shenzhen Key Lab Marine Archaea Geo, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China
3.Southern Marine Sci & Engn Guangdong Lab Guangzhou, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China

Zhang, Jianzhi,Li, Tuo,Hong, Zhilai,et al. Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022.

Zhang, Jianzhi.,Li, Tuo.,Hong, Zhilai.,Ma, Chenfei.,Fang, Xiaoting.,...&Si, Tong.(2022).Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.

Zhang, Jianzhi,et al."Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022).