中文版 | English
Title

Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa

Author
Corresponding AuthorZhao, Chunzhao
Publication Years
2022-12-12
DOI
Source Title
ISSN
1664-462X
Volume13
Abstract
Protochlorophyllide oxidoreductase (POR) plays a key role in catalyzing the light-dependent reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), and thus promotes the transit from etiolated seedlings to green plants. In this study, by exploring ethyl methanesulfonate (EMS)-mediated mutagenesis in Chenopodium quinoa NL-6 variety, we identified a mutant nl6-35 that displays faded green leaf and reduced chlorophyll (Chl) and carotenoid contents. Bulk segregant analysis (BSA) revealed that a mutation in CqPORB gene is genetically associated with the faded green leaf of the nl6-35 mutant. Further study indicates that the nl6-35 mutant exhibits abnormal grana stacks and compromised conversion of Pchlide to Chlide upon illumination, suggesting the important role of CqPORB in producing photoactive Pchlide. Totally three CqPOR isoforms, including CqPORA, CqPORA-like, and CqPORB are identified in NL-6 variety. Transcriptional analysis shows that the expression of all these three CqPOR isoforms is regulated in light- and development-dependent manners, and in mature quinoa plants only CqPORB isoform is predominantly expressed. Subcellular localization analysis indicates that CqPORB is exclusively localized in chloroplast. Together, our study elucidates the important role of CqPORB in the regulation of Chl biosynthesis and chloroplast development in quinoa.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
WOS Research Area
Plant Sciences
WOS Subject
Plant Sciences
WOS Accession No
WOS:000901923600001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/420796
DepartmentInstitute of Advanced Biotechnology
生命科学学院
Affiliation
1.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Bright Agr Dev Grp Co Ltd, Shanghai, Peoples R China
4.Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China
5.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China
6.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen, Peoples R China
Recommended Citation
GB/T 7714
Li, Chao,Ran, Minyuan,Liu, Jianwei,et al. Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa[J]. Frontiers in Plant Science,2022,13.
APA
Li, Chao.,Ran, Minyuan.,Liu, Jianwei.,Wang, Xiaoxiao.,Wu, Qingbing.,...&Zhao, Chunzhao.(2022).Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa.Frontiers in Plant Science,13.
MLA
Li, Chao,et al."Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa".Frontiers in Plant Science 13(2022).
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