Anti-influenza properties of tiliroside isolated from Hibiscus mutabilis L.

Xiao, Mengjie1; Zhang, Tianbo1; Cao, Fei2; Liang, Weihong7,8; Yang, Yinhua5; Huang, Tao9,10; Tang, Yun-Sang1; Liu, Bo7,8; Zhao, Xin6; Shaw, Pang-Chui1,3,4,11

2023-03-01

10.1016/j.jep.2022.115918

JOURNAL OF ETHNOPHARMACOLOGY   Impact Factor & Quartile

0378-8741

1872-7573

Ethnopharmacological relevance: Fu Rong Ye (FRY), the leaf of Hibiscus mutabilis L., is a Chinese medicinal herb used to treat coughs and respiratory diseases. FRY is the major herbal component of the patent medicine Fupo Ganmao Granules for treating common cold. However, its anti-influenza active components and mechanism were not identified.Aim: Here, we aim to a) isolate the anti-influenza phytochemicals from FRY extract and b) explore its anti-flu mechanism.Material and methods: Bioassay guided isolation was performed to get anti-influenza virus components. Influenza virus infected cells and mouse model were employed for efficacy evaluation. Results: Using bioassay-guided isolation, the flavonoid tiliroside was obtained, which inhibited four IAV strains in MDCK cells with EC50 ranging from 3.87 to 27.61 mu M by suppressing the viral ribonucleoprotein activity. Tiliroside also significantly downregulated the expression of cytokines/chemokines in A549 cells, and protected 50% of PR8-infected BALB/c mice from death and at 800 mg/kg/day, improved lung edema conditions.Conclusion: Tiliroside is effective for influenza virus infection treatment and promising for further drug devel-opment. This study is the first to demonstrate that tiliroside in FRY acts against influenza virus.

Hibiscus mutabilis L Influenza virus Ribonucleoprotein

SCI

English

Others

[19180302] ; [XJ2020041] ; [202002010004] ; [2020GDASYL-20200103046]

Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine

Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy

WOS:000897662300004

ELSEVIER IRELAND LTD

PHARMACOLOGY & TOXICOLOGY

2-s2.0-85144636715

Web of Science

1.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong 999077, Peoples R China
2.Hebei Univ, Coll Pharmaceut Sci, Baoding 077000, Peoples R China
3.Chinese Univ Hong Kong, Li Dak Sum Yip Yio Chin R&D Ctr Chinese Med, Shatin, Hong Kong 999077, Peoples R China
4.Chinese Univ Hong Kong, State Key Lab Res Bioact & Clin Applicat Med Plant, Shatin, Hong Kong 999077, Peoples R China
5.Southern Univ Sci & Technol, SUSTech Core Res Facil, Shenzhen 518000, Peoples R China
6.Guangdong Acad Sci, Guangzhou Inst Anal, China Natl Analyt Ctr, Guangzhou 510000, Peoples R China
7.Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangdong Prov Key Lab Clin Res Tradit Chinese Med, Guangzhou 510006, Peoples R China
8.Guangzhou Key Lab Chiral Res Act Components Tradit, Guangzhou 510006, Peoples R China
9.Guangdong Acad Sci, Guangdong Prov Key Lab Chem Measurement, China Natl Analyt Ctr, Guangzhou, Peoples R China
10.Guangdong Acad Sci, Emergency Test Technol Inst Anal, China Natl Analyt Ctr, Guangzhou, CA, Peoples R China
11.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Peoples R China

Xiao, Mengjie,Zhang, Tianbo,Cao, Fei,et al. Anti-influenza properties of tiliroside isolated from Hibiscus mutabilis L.[J]. JOURNAL OF ETHNOPHARMACOLOGY,2023,303.

Xiao, Mengjie.,Zhang, Tianbo.,Cao, Fei.,Liang, Weihong.,Yang, Yinhua.,...&Shaw, Pang-Chui.(2023).Anti-influenza properties of tiliroside isolated from Hibiscus mutabilis L..JOURNAL OF ETHNOPHARMACOLOGY,303.

Xiao, Mengjie,et al."Anti-influenza properties of tiliroside isolated from Hibiscus mutabilis L.".JOURNAL OF ETHNOPHARMACOLOGY 303(2023).