High resolution intravital photoacoustic microscopy reveals VEGF-induced bone regeneration in mouse tibia

Chen, Qian1; Wang, Ziyan1; Yang, Chengyu1; Li, Baochen1; Ren, Xingxing1; Liu, Chao1,2; Xi, Lei1,2,3

2023-02-01

10.1016/j.bone.2022.116631

BONE   Impact Factor & Quartile

8756-3282

1873-2763

Osteogenesis and angiogenesis are essential for bone homeostasis and repair. Newly formed vessels convey osteogenic progenitors during bone regeneration. However, the lack of continuous and label-free visualization of the bone microvasculature has resulted in little understanding of the neovascular dynamics. Here, we take advantage of optical-resolution photoacoustic microscopy (ORPAM) for label-free, intravital, long-term obser-vation of the bone vascular dynamics, including angiogenesis, remodeling and quantified angiogenic effect of locally-applied vascular endothelial growth factor (VEGF) in the murine tibial defect model. We employed ex vivo confocal microscopy and micro-computed tomography (micro-CT) imaging to verify the positive role of VEGF treatment. VEGF treatment increased the concentration of total hemoglobin, vascular branching, and vascular density, which correlated with more osteoprogenitors and increased bone formation within the defect. These data demonstrated ORPAM as a useful imaging tool that detected functional capillaries to understand hemo-dynamics, and revealed the effectiveness of locally delivered therapeutic agents with sufficient sensitivity, contributing to the understanding of spatiotemporal regulatory mechanisms on blood vessels during bone regeneration.

Bone regeneration VEGF Optical-resolution photoacoustic microscopy Angiogenesis Osteogenesis

SCI

English

First ; Corresponding

National Natural Science Foundation of China["62022037","61775028","81571722","61528401"] ; Department of Science and Technology of Guangdong Province["2019ZT08Y191","SZBL2020090501013","2022B1212010003"] ; Department of Education of Guangdong Province[2021ZDZX1064] ; Shenzhen Science and Technology Program["KQTD20190929172743294","JCYJ20200109141 222892","JCYJ20190809114209434","20201022173046002"] ; Shenzhen Science and Technology Innovation Commission[KQTD2020082 0113012029] ; Southern University of Science and Technology["Y01386110","Y01386210"]

Endocrinology & Metabolism

Endocrinology & Metabolism

WOS:000899593700001

ELSEVIER SCIENCE INC

CLINICAL MEDICINE

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China
3.Shenzhen Bay Lab, Shenzhen 518132, Guangdong, Peoples R China

Chen, Qian,Wang, Ziyan,Yang, Chengyu,et al. High resolution intravital photoacoustic microscopy reveals VEGF-induced bone regeneration in mouse tibia[J]. BONE,2023,167.

Chen, Qian.,Wang, Ziyan.,Yang, Chengyu.,Li, Baochen.,Ren, Xingxing.,...&Xi, Lei.(2023).High resolution intravital photoacoustic microscopy reveals VEGF-induced bone regeneration in mouse tibia.BONE,167.

Chen, Qian,et al."High resolution intravital photoacoustic microscopy reveals VEGF-induced bone regeneration in mouse tibia".BONE 167(2023).