High-Contrast Luminescent Immunohistochemistry Using PEGylated Lanthanide Complexes

Su, Fei1,2; Luo, Xiongjian1; Du, Zhongbo1; Chen, Zelyu1; Liu, Yuanhua1; Jin, Xuan1; Guo, Zhiyong1; Lu, Jie1; Jin, Dayong1,2

2022-12-01

10.1021/acs.analchem.2c04058

ANALYTICAL CHEMISTRY   Impact Factor & Quartile

0003-2700

1520-6882

Immunohistochemistry (IHC) using fluorescent probes provides high resolution with multiplexing capability, but the imaging contrast is limited by the brightness of the fluorescent probe and the intrinsic autofluorescence background from tissues. Herein, we improved the contrast by high-density labeling of long-lifetime lanthanide complexes and time-gated imaging. As the large (similar to 280 nm) Stokes shift of lanthanide complexes effectively prevents the issue of concentration quenching, we succeeded in conjugating seven europium complexes to an eight-arm hydrophilic poly(ethylene glycol) (PEG) linker for signal amplification with improved water solubility to the level of up to 10 mg/ mL. Moreover, we demonstrated that both human epidermal growth factor receptor 2 (HER2) in a formalin-fixed paraffin-embedded (FFPE) tissue section and cytokeratin 18 (CK18) in a frozen section can be resolved with the enhanced contrast by 2-fold and 3-fold, respectively. Furthermore, we show that the PEGylation of multiple lanthanide complexes is compatible with tyramide signal amplification (TSA). This work suggests new opportunities for sensitive imaging of low-abundance biomarkers in a tissue matrix.

SCI

English

NI Journal Papers

First ; Corresponding

Shenzhen Science and Technology Program["20200925174735005","KQTD20170810110913065"] ; Australia China Science and Research Fund Joint Research Centre for Point-of-Care Testing["SQ2017YFGH001190","ACSRF658277"] ; Australian Research Council Laureate Fellowship Program[FL210100180]

Chemistry

Chemistry, Analytical

WOS:000893145700001

AMER CHEMICAL SOC

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, UTS SUSTech Joint Res Ctr Biomed Mat & Devices, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Technol Sydney, Inst Biomed Mat & Devices IBMD, Fac Sci, Sydney, NSW 2007, Australia

Su, Fei,Luo, Xiongjian,Du, Zhongbo,et al. High-Contrast Luminescent Immunohistochemistry Using PEGylated Lanthanide Complexes[J]. ANALYTICAL CHEMISTRY,2022.

Su, Fei.,Luo, Xiongjian.,Du, Zhongbo.,Chen, Zelyu.,Liu, Yuanhua.,...&Jin, Dayong.(2022).High-Contrast Luminescent Immunohistochemistry Using PEGylated Lanthanide Complexes.ANALYTICAL CHEMISTRY.

Su, Fei,et al."High-Contrast Luminescent Immunohistochemistry Using PEGylated Lanthanide Complexes".ANALYTICAL CHEMISTRY (2022).