Title | Alkaline stress reduces root waving by regulating PIN7 vacuolar transport |
Author | |
Corresponding Author | Li, Xugang; Li, Ruixi |
Publication Years | 2022-12-13
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DOI | |
Source Title | |
ISSN | 1664-462X
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Volume | 13 |
Abstract | Root development and plasticity are assessed via diverse endogenous and environmental cues, including phytohormones, nutrition, and stress. In this study, we observed that roots in model plant Arabidopsis thaliana exhibited waving and oscillating phenotypes under normal conditions but lost this pattern when subjected to alkaline stress. We later showed that alkaline treatment disturbed the auxin gradient in roots and increased auxin signal in columella cells. We further demonstrated that the auxin efflux transporter PIN-FORMED 7 (PIN7) but not PIN3 was translocated to vacuole lumen under alkaline stress. This process is essential for root response to alkaline stress because the pin7 knockout mutants retained the root waving phenotype. Moreover, we provided evidence that the PIN7 vacuolar transport might not depend on the ARF-GEFs but required the proper function of an ESCRT subunit known as FYVE domain protein required for endosomal sorting 1 (FREE1). Induced silencing of FREE1 disrupted the vacuolar transport of PIN7 and reduced sensitivity to alkaline stress, further highlighting the importance of this cellular process. In conclusion, our work reveals a new role of PIN7 in regulating root morphology under alkaline stress. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | Corresponding
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WOS Research Area | Plant Sciences
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WOS Subject | Plant Sciences
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WOS Accession No | WOS:000902556700001
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Publisher | |
Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:1
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/420816 |
Department | SUSTech-Peking University National Institute of Plants and Food 生命科学学院 生命科学学院_生物系 |
Affiliation | 1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An, Peoples R China 2.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory Guangdong Hi, Inst Plant & Food Sci, Sch Life Sci, Shenzhen, Peoples R China 3.Shandong Agr Univ, Coll Life Sci, Sino German Joint Res Ctr Agr Biol, Tai An, Peoples R China 4.Northeast Forestry Univ, Coll Life Sci, Key Lab Saline alkali Vegetat Ecol Restorat, Minist Educ, Harbin, Peoples R China 5.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, MOA Key Lab Plant Nutr & Fertilizat Lowermiddle Re, Nanjing, Peoples R China 6.Albert Ludwigs Univ Freiburg, Inst Biol II Mol Plant Physiol, Fac Biol, Schanzlestr 1, Freiburg, Germany |
First Author Affilication | SUSTech-Peking University National Institute of Plants and Food; Department of Biology; School of Life Sciences |
Corresponding Author Affilication | SUSTech-Peking University National Institute of Plants and Food; Department of Biology; School of Life Sciences |
Recommended Citation GB/T 7714 |
Liu, Yu,Mu, Chenglin,Du, Dongdong,et al. Alkaline stress reduces root waving by regulating PIN7 vacuolar transport[J]. Frontiers in Plant Science,2022,13.
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APA |
Liu, Yu.,Mu, Chenglin.,Du, Dongdong.,Yang, Yi.,Li, Lixin.,...&Li, Ruixi.(2022).Alkaline stress reduces root waving by regulating PIN7 vacuolar transport.Frontiers in Plant Science,13.
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MLA |
Liu, Yu,et al."Alkaline stress reduces root waving by regulating PIN7 vacuolar transport".Frontiers in Plant Science 13(2022).
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