中文版 | English
Title

Alkaline stress reduces root waving by regulating PIN7 vacuolar transport

Author
Corresponding AuthorLi, Xugang; Li, Ruixi
Publication Years
2022-12-13
DOI
Source Title
ISSN
1664-462X
Volume13
Abstract

Root development and plasticity are assessed via diverse endogenous and environmental cues, including phytohormones, nutrition, and stress. In this study, we observed that roots in model plant Arabidopsis thaliana exhibited waving and oscillating phenotypes under normal conditions but lost this pattern when subjected to alkaline stress. We later showed that alkaline treatment disturbed the auxin gradient in roots and increased auxin signal in columella cells. We further demonstrated that the auxin efflux transporter PIN-FORMED 7 (PIN7) but not PIN3 was translocated to vacuole lumen under alkaline stress. This process is essential for root response to alkaline stress because the pin7 knockout mutants retained the root waving phenotype. Moreover, we provided evidence that the PIN7 vacuolar transport might not depend on the ARF-GEFs but required the proper function of an ESCRT subunit known as FYVE domain protein required for endosomal sorting 1 (FREE1). Induced silencing of FREE1 disrupted the vacuolar transport of PIN7 and reduced sensitivity to alkaline stress, further highlighting the importance of this cellular process. In conclusion, our work reveals a new role of PIN7 in regulating root morphology under alkaline stress.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
WOS Research Area
Plant Sciences
WOS Subject
Plant Sciences
WOS Accession No
WOS:000902556700001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/420816
DepartmentSUSTech-Peking University National Institute of Plants and Food
生命科学学院
生命科学学院_生物系
Affiliation
1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An, Peoples R China
2.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory Guangdong Hi, Inst Plant & Food Sci, Sch Life Sci, Shenzhen, Peoples R China
3.Shandong Agr Univ, Coll Life Sci, Sino German Joint Res Ctr Agr Biol, Tai An, Peoples R China
4.Northeast Forestry Univ, Coll Life Sci, Key Lab Saline alkali Vegetat Ecol Restorat, Minist Educ, Harbin, Peoples R China
5.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, MOA Key Lab Plant Nutr & Fertilizat Lowermiddle Re, Nanjing, Peoples R China
6.Albert Ludwigs Univ Freiburg, Inst Biol II Mol Plant Physiol, Fac Biol, Schanzlestr 1, Freiburg, Germany
First Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Corresponding Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Liu, Yu,Mu, Chenglin,Du, Dongdong,et al. Alkaline stress reduces root waving by regulating PIN7 vacuolar transport[J]. Frontiers in Plant Science,2022,13.
APA
Liu, Yu.,Mu, Chenglin.,Du, Dongdong.,Yang, Yi.,Li, Lixin.,...&Li, Ruixi.(2022).Alkaline stress reduces root waving by regulating PIN7 vacuolar transport.Frontiers in Plant Science,13.
MLA
Liu, Yu,et al."Alkaline stress reduces root waving by regulating PIN7 vacuolar transport".Frontiers in Plant Science 13(2022).
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