中文版 | English
Title

Measuring capillary flow dynamics using interlaced two-photon volumetric scanning

Author
Corresponding AuthorGiblin, John T.
Publication Years
2022-12-01
DOI
Source Title
ISSN
0271-678X
EISSN
1559-7016
Volume43Issue:4Pages:595-609
Abstract
Two photon microscopy and optical coherence tomography (OCT) are two standard methods for measuring flow speeds of red blood cells in microvessels, particularly in animal models. However, traditional two photon microscopy lacks the depth of field to adequately capture the full volumetric complexity of the cerebral microvasculature and OCT lacks the specificity offered by fluorescent labeling. In addition, the traditional raster scanning technique utilized in both modalities requires a balance of image frame rate and field of view, which severely limits the study of RBC velocities in the microvascular network. Here, we overcome this by using a custom two photon system with an axicon based Bessel beam to obtain volumetric images of the microvascular network with fluorescent specificity. We combine this with a novel scan pattern that generates pairs of frames with short time delay sufficient for tracking red blood cell flow in capillaries. We track RBC flow speeds in 10 or more capillaries simultaneously at 1 Hz in a 237 mu m x 237 mu m x 120 mu m volume and quantified both their spatial and temporal variability in speed. We also demonstrate the ability to track flow speed changes around stalls in capillary flow and measure to 300 mu m in depth.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
WOS Research Area
Endocrinology & Metabolism ; Hematology ; Neurosciences & Neurology
WOS Subject
Endocrinology & Metabolism ; Hematology ; Neurosciences
WOS Accession No
WOS:000895977500001
Publisher
ESI Research Field
NEUROSCIENCE & BEHAVIOR
Scopus EID
2-s2.0-85144179859
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:2
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/420822
DepartmentDepartment of Biomedical Engineering
Affiliation
1.Boston Univ, Neurophoton Ctr, Dept Biomed Engn, Boston, MA USA
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Peoples R China
Recommended Citation
GB/T 7714
Giblin, John T.,Park, Seong-Wook,Jiang, John,et al. Measuring capillary flow dynamics using interlaced two-photon volumetric scanning[J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM,2022,43(4):595-609.
APA
Giblin, John T..,Park, Seong-Wook.,Jiang, John.,Kilic, Kivilcim.,Kura, Sreekanth.,...&Chen, Ichun A..(2022).Measuring capillary flow dynamics using interlaced two-photon volumetric scanning.JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM,43(4),595-609.
MLA
Giblin, John T.,et al."Measuring capillary flow dynamics using interlaced two-photon volumetric scanning".JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 43.4(2022):595-609.
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