Measuring capillary flow dynamics using interlaced two-photon volumetric scanning

Giblin, John T.1; Park, Seong-Wook1; Jiang, John1; Kilic, Kivilcim1; Kura, Sreekanth1; Tang, Jianbo2; Boas, David A.1; Chen, Ichun A.1

2022-12-01

10.1177/0271678X221145091

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM   Impact Factor & Quartile

0271-678X

1559-7016

Two photon microscopy and optical coherence tomography (OCT) are two standard methods for measuring flow speeds of red blood cells in microvessels, particularly in animal models. However, traditional two photon microscopy lacks the depth of field to adequately capture the full volumetric complexity of the cerebral microvasculature and OCT lacks the specificity offered by fluorescent labeling. In addition, the traditional raster scanning technique utilized in both modalities requires a balance of image frame rate and field of view, which severely limits the study of RBC velocities in the microvascular network. Here, we overcome this by using a custom two photon system with an axicon based Bessel beam to obtain volumetric images of the microvascular network with fluorescent specificity. We combine this with a novel scan pattern that generates pairs of frames with short time delay sufficient for tracking red blood cell flow in capillaries. We track RBC flow speeds in 10 or more capillaries simultaneously at 1 Hz in a 237 mu m x 237 mu m x 120 mu m volume and quantified both their spatial and temporal variability in speed. We also demonstrate the ability to track flow speed changes around stalls in capillary flow and measure to 300 mu m in depth.

Blood flow capillary flow heterogeneity stall two-photon microscopy

SCI

English

Others

Endocrinology & Metabolism ; Hematology ; Neurosciences & Neurology

Endocrinology & Metabolism ; Hematology ; Neurosciences

WOS:000895977500001

SAGE PUBLICATIONS INC

NEUROSCIENCE & BEHAVIOR

2-s2.0-85144179859

Web of Science

1.Boston Univ, Neurophoton Ctr, Dept Biomed Engn, Boston, MA USA
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Peoples R China

Giblin, John T.,Park, Seong-Wook,Jiang, John,et al. Measuring capillary flow dynamics using interlaced two-photon volumetric scanning[J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM,2022,43(4):595-609.

Giblin, John T..,Park, Seong-Wook.,Jiang, John.,Kilic, Kivilcim.,Kura, Sreekanth.,...&Chen, Ichun A..(2022).Measuring capillary flow dynamics using interlaced two-photon volumetric scanning.JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM,43(4),595-609.

Giblin, John T.,et al."Measuring capillary flow dynamics using interlaced two-photon volumetric scanning".JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM 43.4(2022):595-609.