| Title | Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics |
| Author | |
| Corresponding Author | Wang, Dou; Jiang, Xingyu |
| Publication Years | 2023
|
| DOI | |
| Source Title | |
| ISSN | 0165-9936
|
| EISSN | 1879-3142
|
| Volume | 158 |
| Abstract | The coronavirus disease 2019 (COVID-19) has extensively promoted the application of nucleic acid testing technology in the field of clinical testing. The most widely used polymerase chain reaction (PCR)-based nucleic acid testing technology has problems such as complex operation, high requirements of personnel and laboratories, and contamination. The highly miniaturized microfluidic chip provides an essential tool for integrating the complex nucleic acid detection process. Various microfluidic chips have been developed for the rapid detection of nucleic acid, such as amplification-free microfluidics in combination with clustered regularly interspaced short palindromic repeats (CRISPR). In this review, we first summarized the routine process of nucleic acid testing, including sample processing and nucleic acid detection. Then the typical microfluidic chip technologies and new research advances are sum-marized. We also discuss the main problems of nucleic acid detection and the future developing trend of the microfluidic chip.(c) 2022 Elsevier B.V. All rights reserved. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | National Key R&D Program of China["2020YFA0908900","2021YFF1200100"]
; National Natural Science Foundation of China["21761142006","21535001","22204068","81730051"]
; Shenzhen Science and Technology Program["JCYJ20210324105006017","KQTD20190929172743294","KCXFZ20211020163544002"]
; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009]
; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]
; Guangdong Basic and Applied Basic Research Foundation[2019A1515110292]
; Chinese Academy of Sciences[Q YZDJ-SSW-SLH039]
; Shenzhen Bay Laboratory[SZBL2019062801004]
|
| WOS Research Area | Chemistry
|
| WOS Subject | Chemistry, Analytical
|
| WOS Accession No | WOS:000900061900010
|
| Publisher | |
| ESI Research Field | CHEMISTRY
|
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:1
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/424599 |
| Department | Department of Biomedical Engineering |
| Affiliation | Southern Univ Sci & Technol, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China |
| First Author Affilication | Department of Biomedical Engineering |
| Corresponding Author Affilication | Department of Biomedical Engineering |
| First Author's First Affilication | Department of Biomedical Engineering |
| Recommended Citation GB/T 7714 |
Li, Zheng,Xu, Xiaojian,Wang, Dou,et al. Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2023,158.
|
| APA |
Li, Zheng,Xu, Xiaojian,Wang, Dou,&Jiang, Xingyu.(2023).Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,158.
|
| MLA |
Li, Zheng,et al."Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 158(2023).
|
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