中文版 | English
Title

VgrG Spike Dictates PAAR Requirement for the Assembly of the Type VI Secretion System

Author
Corresponding AuthorDong, Tao
Publication Years
2023-01-19
DOI
Source Title
ISSN
0021-9193
EISSN
1098-5530
Volume205Issue:2
Abstract

["Gram-negative bacteria often encode multiple paralogs of the cone-shaped PAAR that sits atop the VgrG-spike and is thought to sharpen the spear-like T6SS puncturing device. However, it is unclear why PAAR is required for the assembly of some but not all T6SSs and why there are multiple PAARs if they are not required.","Widely employed by Gram-negative pathogens for competition and pathogenesis, the type six protein secretion system (T6SS) can inject toxic effectors into neighboring cells through the penetration of a spear-like structure comprising a long Hcp tube and a VgrG-PAAR spike complex. The cone-shaped PAAR is believed to sharpen the T6SS spear for penetration but it remains unclear why PAAR is required for T6SS functions in some bacteria but dispensable in others. Here, we report the conditional requirement of PAAR for T6SS functions in Aeromonas dhakensis, an emerging human pathogen that may cause severe bacteremia. By deleting the two PAAR paralogs, we show that PAAR is not required for T6SS secretion, bacterial killing, or specific effector delivery in A. dhakensis. By constructing combinatorial PAAR and vgrG deletions, we demonstrate that deletion of individual PAAR moderately reduced T6SS functions but double or triple deletions of PAAR in the vgrG deletion mutants severely impaired T6SS functions. Notably, the auxiliary-cluster-encoded PAAR2 and VgrG3 are less critical than the main-cluster-encoded PAAR1 and VgrG1&2 proteins to T6SS functions. In addition, PAAR1 but not PAAR2 contributes to antieukaryotic virulence in amoeba. Our data suggest that, for a multi-PAAR T6SS, the variable role of PAAR paralogs correlates with the VgrG-spike composition that collectively dictates T6SS assembly.IMPORTANCE Gram-negative bacteria often encode multiple paralogs of the cone-shaped PAAR that sits atop the VgrG-spike and is thought to sharpen the spear-like T6SS puncturing device. However, it is unclear why PAAR is required for the assembly of some but not all T6SSs and why there are multiple PAARs if they are not required. Our data delineate a VgrG-mediated conditional requirement for PAAR and suggest a core-auxiliary relationship among different PAAR-VgrG modules that may have been acquired sequentially by the T6SS during evolution."]

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Key R and D Program of China[
WOS Research Area
Microbiology
WOS Subject
Microbiology
WOS Accession No
WOS:000915808800001
Publisher
ESI Research Field
MICROBIOLOGY
Scopus EID
2-s2.0-85148965562
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/425123
DepartmentSchool of Life Sciences
生命科学学院_基础免疫与微生物学系
Affiliation
1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Shanghai, Peoples R China
2.Southern Univ Sci & Technol, Sch Life Sci, Dept Immunol & Microbiol, Shenzhen, Peoples R China
Corresponding Author AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Liang, Xiaoye,Zheng, Hao-Yu,Zhao, Ya-Jie,et al. VgrG Spike Dictates PAAR Requirement for the Assembly of the Type VI Secretion System[J]. JOURNAL OF BACTERIOLOGY,2023,205(2).
APA
Liang, Xiaoye.,Zheng, Hao-Yu.,Zhao, Ya-Jie.,Zhang, Yi-Qiu.,Pei, Tong-Tong.,...&Dong, Tao.(2023).VgrG Spike Dictates PAAR Requirement for the Assembly of the Type VI Secretion System.JOURNAL OF BACTERIOLOGY,205(2).
MLA
Liang, Xiaoye,et al."VgrG Spike Dictates PAAR Requirement for the Assembly of the Type VI Secretion System".JOURNAL OF BACTERIOLOGY 205.2(2023).
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