中文版 | English
Title

SYNTAXIN OF PLANTS81 regulates root meristem activity and stem cell niche maintenance via ROS signaling

Author
Corresponding AuthorLi, Lixin
Publication Years
2022-11-01
DOI
Source Title
ISSN
0032-0889
EISSN
1532-2548
Abstract
Root growth and development depend on continuous cell division and differentiation in root tips. In these processes, reactive oxygen species (ROS) play a critical role as signaling molecules. However, few ROS signaling regulators have been identified. In this study, we found knockdown of a syntaxin gene, SYNTAXIN OF PLANTS81 in Arabidopsis thaliana (AtSYP81) resulted in a severe reduction in root meristem activity and disruption of root stem cell niche (SCN) identity. Subsequently, we found AtSYP81 was highly expressed in roots and localized on the endoplasmic reticulum (ER). Interestingly, the reduced expression of AtSYP81 conferred a decreased number of peroxisomes in root meristem cells, raising a possibility that AtSYP81 regulates root development through peroxisome-mediated ROS production. Further transcriptome analysis revealed that class III peroxidases, which are responsible for intracellular ROS homeostasis, showed significantly changed expression in the atsyp81 mutants and AtSYP81 overexpression lines, adding evidence of the regulatory role of AtSYP81 in ROS signaling. Accordingly, rescuing the decreased ROS level via applying ROS donors effectively restored the defects in root meristem activity and SCN identity in the atsyp81 mutants. APETALA2 (AP2) transcription factors PLETHORA1 and 2 (PLT1 and PLT2) were then established as the downstream effectors in this pathway, while potential crosstalk between ROS signaling and auxin signaling was also indicated. Taken together, our findings suggest that AtSYP81 regulates root meristem activity and maintains root SCN identity by controlling peroxisome- and peroxidase-mediated ROS homeostasis, thus both broadening and deepening our understanding of the biological roles of SNARE proteins and ROS signaling.
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
National Natural Science Foundation of China["32170279","31570246"] ; Fundamental Research Funds for the Central Universities[2572019CT03]
WOS Research Area
Plant Sciences
WOS Subject
Plant Sciences
WOS Accession No
WOS:000914578800001
Publisher
ESI Research Field
PLANT & ANIMAL SCIENCE
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/425146
DepartmentInstitute of Advanced Biotechnology
生命科学学院
Affiliation
1.Northeast Forestry Univ, Coll Life Sci, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Peoples R China
2.Peking Univ, Inst Adv Agr Sci, Shandong Lab Adv Agr Sci Weifang, Weifang 261000, Shandong, Peoples R China
3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Changchun 130102, Peoples R China
4.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen 518055, Peoples R China
5.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China
6.Chinese Acad Agr Sci, Ctr Adv Bioind Technol, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Wang, Mingjing,Zhang, Hailong,Zhao, Xiaonan,et al. SYNTAXIN OF PLANTS81 regulates root meristem activity and stem cell niche maintenance via ROS signaling[J]. PLANT PHYSIOLOGY,2022.
APA
Wang, Mingjing.,Zhang, Hailong.,Zhao, Xiaonan.,Zhou, Jingwen.,Qin, Guochen.,...&Li, Lixin.(2022).SYNTAXIN OF PLANTS81 regulates root meristem activity and stem cell niche maintenance via ROS signaling.PLANT PHYSIOLOGY.
MLA
Wang, Mingjing,et al."SYNTAXIN OF PLANTS81 regulates root meristem activity and stem cell niche maintenance via ROS signaling".PLANT PHYSIOLOGY (2022).
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