中文版 | English
Title

Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification

Author
Corresponding AuthorPeng, Jinrong; Gao, Ce
Publication Years
2022-12-01
DOI
Source Title
ISSN
1673-8527
EISSN
1873-5533
Volume49Issue:12
Abstract
In mammals, the expression of the homeobox family member Cdx2/CDX2 is restricted within the intestine. Conditional ablation of the mouse Cdx2 in the endodermal cells causes a homeotic transformation of the intestine towards the esophagus or gastric fate. In this report, we show that null mutants of zebrafish cdx1b, encoding the counterpart of mammalian CDX2, could survive more than 10 days post fertilization, a stage when the zebrafish digestive system has been well developed. Through RNA sequencing (RNA-seq) and single-cell sequencing (scRNA-seq) of the dissected intestine from the mutant embryos, we demonstrate that the loss-of-function of the zebrafish cdx1b yields hepatocyte-like intestinal cells, a phenotype never observed in the mouse model. Further RNA-seq data analysis, and genetic double mutants and signaling inhibitor studies reveal that Cdx1b functions to guard the intestinal fate by repressing, directly or indirectly, a range of transcriptional factors and signaling pathways for liver specification. Finally, we demonstrate that heat shock-induced overexpression of cdx1b in a transgenic fish abolishes the liver formation. Therefore, we demonstrate that Cdx1b is a key repressor of hepatic fate during the intestine specification in zebrafish.Copyright (c) 2022, The Authors. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
National Key R&D Program of China[2018YFA0800502] ; National Natural Science Foundation of China["31900579","31830113"]
WOS Research Area
Biochemistry & Molecular Biology ; Genetics & Heredity
WOS Subject
Biochemistry & Molecular Biology ; Genetics & Heredity
WOS Accession No
WOS:000917286300001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/430730
DepartmentSchool of Medicine
Affiliation
1.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Southern Univ Sci & Technol, Sch Med, Dept Human Cell Biol & Genet, Shenzhen 518055, Guangdong, Peoples R China
3.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Jin, Qingxia,Gao, Yuqi,Shuai, Shimin,et al. Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification[J]. Journal of Genetics and Genomics,2022,49(12).
APA
Jin, Qingxia.,Gao, Yuqi.,Shuai, Shimin.,Chen, Yayue.,Wang, Kaiyuan.,...&Gao, Ce.(2022).Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification.Journal of Genetics and Genomics,49(12).
MLA
Jin, Qingxia,et al."Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification".Journal of Genetics and Genomics 49.12(2022).
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